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| Vector delivery is still a bottleneck for gene therapy. To overcome some disadvantages of adenoviral and retroviral vectors, we developed a hybrid vector. This hybrid vector, AdLTR-luc, was created by adding two elements from Moloney murine leukemia virus (MoMLV) flanking the luciferase cDNA into an E1/E3-deleted, replication deficient serotype 5 adenovirus vector (Zheng et al., Nature Biotechnol, 2000), and demonstrated that the MoMLV element upstream of the luciferase cDNA was broken during the integration event. The purpose of the current study was to determine if the MoMLV element downstream of the luciferase cDNA was also broken when integration occurred. We used the same A5 cell clones (#10 and 11) from the earlier the paper along with restriction endonuclease digestions, plus Southern hybridization, and PCR. Southern hybridization indicated that the luciferase cDNA was intact in the cloned cells. Results from Xho I and Sal I digestions showed that integration occurred in cloned cells. ...details |
| Vascular endothelial growth factor (VEGF) is essential for normal vascular growth and development during wound repair. VEGF is estrogen responsive and capable of regulating its own receptor, vascular endothelial growth factor receptor-2 (VEGFR-2). Several agricultural pesticides (e.g., methoxychlor) have estrogenic potential that can initiate inappropriate physiological responses in estrogenic-sensitive tissues following exposure in vivo. Thus, the current study was designed to determine whether the VEGFR-2-Luciferase (Luc) reporter transgenic mouse is a useful model for evaluating estrogenic tendencies of methoxychlor by monitoring wound healing via VEGFR-2-mediated gene expression using bioluminescence and real-time imaging technology. ...details |
| A Human Head Surrogate has been developed for use in behind helmet blunt trauma experiments. This human head surrogate fills the void between Post-Mortem Human Subject testing (with biofidelity but handling restrictions) and commercial ballistic head forms (with no biofidelity but ease of use). This unique human head surrogate is based on refreshed human craniums and surrogate materials representing human head soft tissues such as the skin, dura, and brain. A methodology for refreshing the craniums is developed and verified through material testing. ...details |
| Study Design: A randomized, double-blind, active-controlled trial. Objective: To assess the effectiveness of cervical interlaminar epidural injections of local anesthetic with or without steroids for the management of axial or discogenic pain in patients without disc herniation, radiculitis, or facet joint pain. Summary of Background Data: Cervical discogenic pain without disc herniation is a common cause of suffering and disability in the adult population. Once conservative management has failed and facet joint pain has been excluded, cervical epidural injections may be considered as a management tool. Despite a paucity of evidence, cervical epidural injections are one of the most commonly performed nonsurgical interventions in the management of chronic axial or disc-related neck pain....details |
 Review
Characterization of the Asian Phenotype - An Emerging Paradigm with Clinicopathological and Human Research Implications
Melvin Khee-Shing Leow
Int. J. Med. Sci. 2017; 14(7): 639-647. doi:10.7150/ijms.18880
[Abstract]
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Involvement of Ornithine Carbamoyltransferase in the Progression of Chronic Hepatitis C and Liver Cirrhosis
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MicroRNA Expression Analysis of Centenarians and Rheumatoid Arthritis Patients Reveals a Common Expression Pattern
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Systematic Evaluation of Corticosteroid Use in Obese and Non-obese Individuals: A Multi-cohort Study
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Titanium Dioxide Nanoparticles as Radiosensitisers: An In vitro and Phantom-Based Study
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Gastric Juice-Based Real-Time PCR for Tailored Helicobacter Pylori Treatment: A Practical Approach
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Int. J. Med. Sci. 2017; 14(6): 595-601. doi:10.7150/ijms.18996
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The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders
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Hypoxia-regulated MicroRNA-210 Overexpression is Associated with Tumor Development and Progression in Upper Tract Urothelial Carcinoma
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Int. J. Med. Sci. 2017; 14(6): 578-584. doi:10.7150/ijms.15699
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The Effect of Nefopam Infusion during Laparascopic Cholecystectomy on Postoperative Pain
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Sung-Suk Suh, Eun Jin Yang, Sung Gu Lee, Ui Joung Youn, Se Jong Han, Il-Chan Kim, Sanghee Kim
Int. J. Med. Sci. 2017; 14(6): 560-569. doi:10.7150/ijms.18702
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ProEx C as Diagnostic Marker for Detection of Urothelial Carcinoma in Urinary Samples: A Review
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Int. J. Med. Sci. 2017; 14(6): 554-559. doi:10.7150/ijms.17890
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Existence of a Strong Correlation of Biomarkers and miRNA in Females with Metabolic Syndrome and Obesity in a Population of West Virginia
Perrine Goguet-Rubio, Rebecca L. Klug, Dana L. Sharma, Krithika Srikanthan, Nitin Puri, Vishal H Lakhani, Alexandra Nichols, Kathleen M. O'Hanlon, Nader G. Abraham, Joseph I. Shapiro, Komal Sodhi
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Clinical insomnia and associated factors in failed back surgery syndrome: a retrospective cross-sectional study
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Int. J. Med. Sci. 2017; 14(6): 536-542. doi:10.7150/ijms.18926
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KLF6 inhibited oral cancer migration and invasion via downregulation of mesenchymal markers and inhibition of MMP-9 activities
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Int. J. Med. Sci. 2017; 14(6): 530-535. doi:10.7150/ijms.19024
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Synergistic Antitumor Effect of Sorafenib in Combination with ATM Inhibitor in Hepatocellular Carcinoma Cells
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Int. J. Med. Sci. 2017; 14(6): 523-529. doi:10.7150/ijms.19033
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Nuclear Expression of GS28 Protein: A Novel Biomarker that Predicts Prognosis in Colorectal Cancers
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Int. J. Med. Sci. 2017; 14(6): 515-522. doi:10.7150/ijms.19368
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