International Journal of Medical Sciences

22 December 2014

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Int J Med Sci 2013; 10(4):362-376. doi:10.7150/ijms.5698

Research Paper

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

Eva Sapi1,2✉, Namrata Pabbati1, Akshita Datar1, Ellen M Davies1, Amy Rattelle1, Bruce A Kuo1

1. Research Division of Advanced Laboratory Services Philadelphia PA, USA;
2. Department of Biology and Environmental Science, University of New Haven, West Haven CT, USA.

Abstract

In this report we present a method to cultivate Borrelia spirochetes from human serum samples with high efficiency. This method incorporates improved sample collection, optimization of culture media and use of matrix protein. The method was first optimized utilizing Borrelia laboratory strains, and later by demonstrating growth of Borrelia from sera from fifty seropositive Lyme disease patients followed by another cohort of 72 Lyme disease patients, all of whom satisfied the strict CDC surveillance case definition for Lyme disease. The procedure resulted in positive cultures in 47% at 6 days and 94% at week 16. Negative controls included 48 cases. The positive identification of Borrelia was performed by immunostaining, PCR, and direct DNA sequencing.

Keywords: Clinical microbiology, Vector-borne diseases, Lyme disease, Spirochetes.

How to cite this article:
Sapi E, Pabbati N, Datar A, Davies EM, Rattelle A, Kuo BA. Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum. Int J Med Sci 2013; 10(4):362-376. doi:10.7150/ijms.5698. Available from http://www.medsci.org/v10p0362.htm