19 February 2018
Int J Med Sci 2005; 2(4):122-128. doi:10.7150/ijms.2.122
Differential gene expression in HIV/SIV-associated and spontaneous lymphomas
1. Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq. 2, Moscow, Russia
Diffuse large B-cell lymphoma (DLBCL) is more prevalent and more often fatal in HIV-infected patients and SIV-infected monkeys compared to immune-competent individuals. Molecular, biological, and immunological data indicate that virus-associated lymphomagenesis is similar in both infected hosts. To find genes specifically overexpressed in HIV/SIV-associated and non-HIV/SIV-associated DLBCL we compared gene expression profiles of HIV/SIV-related and non-HIV-related lymphomas using subtractive hybridization and Northern blot analysis. Our experimental approach allowed us to detect two genes (a-myb and pub) upregulated solely in HIV/SIV-associated DLBCLs potentially involved in virus-specific lymphomagenesis in human and monkey. Downregulation of the pub gene was observed in all non-HIV-associated lymphomas investigated. In addition, we have found genes upregulated in both non-HIV- and HIV-associated lymphomas. Among those were genes both with known (set, ND4, SMG-1) and unknown functions. In summary, we have demonstrated that simultaneous transcriptional upregulation of at least two genes (a-myb and pub) was specific for AIDS-associated lymphomas.
Keywords: non-Hodgkin's lymphoma, diffuse large B-cell lymphoma, HIV/SIV-associated lymphomas, spontaneous, differentially expressed genes, subtractive hybridization
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How to cite this article:
Nenasheva VV, Nikolaev AI, Martynenko A, Kaplanskaya IB, Bodemer W, Hunsmann G, Tarantul VZ. Differential gene expression in HIV/SIV-associated and spontaneous lymphomas. Int J Med Sci 2005; 2(4):122-128. doi:10.7150/ijms.2.122. Available from http://www.medsci.org/v02p0122.htm