Int J Med Sci 2022; 19(13):1879-1887. doi:10.7150/ijms.77729 This issue Cite

Research Paper

DPA promotes hBMSCs osteogenic differentiation by miR-9-5p/ERK/ALP signaling pathway

Xiang Gao1#, Guanhao Hong1,2#, Weiqiang Zhan1,2, Tianfeng Liu2, Shouquan Yan1,3, Mingzhu Deng1,2, Chenlin Tu1,2, Peng Li1✉

1. Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
2. Orthopedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China.
3. Scientific Research Department, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China.
#Equal contributions to this work.

Citation:
Gao X, Hong G, Zhan W, Liu T, Yan S, Deng M, Tu C, Li P. DPA promotes hBMSCs osteogenic differentiation by miR-9-5p/ERK/ALP signaling pathway. Int J Med Sci 2022; 19(13):1879-1887. doi:10.7150/ijms.77729. https://www.medsci.org/v19p1879.htm
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Abstract

Graphic abstract

Docosahexaenoic acid (DHA) has been reported potentiate osteogenic differentiation, while Docosapentaenoic acid (DPA), another Omega-3 fatty acid, its contribution to the osteogenic differentiation of human bone-marrow-derived mesenchymal stromal cells (hBMSCs) is not entirely elucidated. The Alizarin Red S (ARS) staining and the expression of osteogenesis‑associated genes were analyzed during osteogenic induction by DPA. Then, bioinformatics analysis and dual luciferase reporter assays were investigated to confirm the interactions between miR-9-5p and alkaline phosphatase (ALP). miR-9-5p mimics / inhibitor were transfected to human hBMSCs and the osteogenic assay above was also performed. Furthermore, DPA significantly promoted the phosphorylation of ERK via miR-9-5p. PD98059, a highly specific and potent ERK1/2 inhibitor, inhibited the activation of ALP and partially reversed the role of DPA during osteogenic differentiation. These data indicated that DPA promoted osteogenic differentiation of hBMSCs potentially through miR-9-5p/ERK/ALP signaling pathway, providing a potentially useful therapeutic strategy for patients to improve bone loss.

Keywords: DPA, osteogenic differentiation, hBMSCs, miR-9-5p, ERK, ALP


Citation styles

APA
Gao, X., Hong, G., Zhan, W., Liu, T., Yan, S., Deng, M., Tu, C., Li, P. (2022). DPA promotes hBMSCs osteogenic differentiation by miR-9-5p/ERK/ALP signaling pathway. International Journal of Medical Sciences, 19(13), 1879-1887. https://doi.org/10.7150/ijms.77729.

ACS
Gao, X.; Hong, G.; Zhan, W.; Liu, T.; Yan, S.; Deng, M.; Tu, C.; Li, P. DPA promotes hBMSCs osteogenic differentiation by miR-9-5p/ERK/ALP signaling pathway. Int. J. Med. Sci. 2022, 19 (13), 1879-1887. DOI: 10.7150/ijms.77729.

NLM
Gao X, Hong G, Zhan W, Liu T, Yan S, Deng M, Tu C, Li P. DPA promotes hBMSCs osteogenic differentiation by miR-9-5p/ERK/ALP signaling pathway. Int J Med Sci 2022; 19(13):1879-1887. doi:10.7150/ijms.77729. https://www.medsci.org/v19p1879.htm

CSE
Gao X, Hong G, Zhan W, Liu T, Yan S, Deng M, Tu C, Li P. 2022. DPA promotes hBMSCs osteogenic differentiation by miR-9-5p/ERK/ALP signaling pathway. Int J Med Sci. 19(13):1879-1887.

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