Int J Med Sci 2022; 19(9):1377-1387. doi:10.7150/ijms.69501 This issue

Research Paper

Hsa_circ_0087302, a circular RNA, affects the progression of osteosarcoma via the Wnt/β-catenin signaling pathway

Lijiao Peng2*, Qianzheng Liu1*, Tingrui Wu1*, Peng Li3, Yixia Cai2, Xinjian Wei1, Yuming Zeng1, Junhong Ye1, Peicong Chen1, Jing Huang2✉, Hao Lin1✉

1. Department of Orthopedics, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, China.
2. Oncology Hospital, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, China.
3. Stem Cell Research and Cellular Therapy Center, Affifiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, China.
*These authors contributed equally to this work.

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Citation:
Peng L, Liu Q, Wu T, Li P, Cai Y, Wei X, Zeng Y, Ye J, Chen P, Huang J, Lin H. Hsa_circ_0087302, a circular RNA, affects the progression of osteosarcoma via the Wnt/β-catenin signaling pathway. Int J Med Sci 2022; 19(9):1377-1387. doi:10.7150/ijms.69501. Available from https://www.medsci.org/v19p1377.htm

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Abstract

Graphic abstract

Osteosarcoma is the most common malignant tumor in adolescent bone malignancies. It has the characteristics of a high metastasis rate, high mortality and poor prognosis. As a subclass of endogenous noncoding RNAs, circRNAs have been identified to be related to the occurrence, development and prognosis of different kinds of cancers, but the mechanism of their effect on osteosarcoma is not clear. In the present study, we identified a novel circRNA, hsa_circ_0087302, by RNA-seq, and we found that it was expressed at low levels in osteosarcoma. Using RT-PCR, we confirmed that the expression of hsa_circ_0087302 in osteosarcoma cells was lower than that in osteoblasts. Functional validation experiments revealed that hsa_circ_0087302 overexpression inhibited proliferation, cell cycle, migration, and invasion in osteosarcoma cells. Furthermore, Western blotting experiments demonstrated that hsa_circ_0087302 affected the expression of cell cycle- and Wnt/β-catenin signaling pathway-related proteins. For the first time, we identified that hsa_circ_0087302 may affect the malignant biological behavior of osteosarcoma cells through the Wnt/β-catenin signaling pathway. In summary, hsa_circ_0087302 may provide a new direction for the diagnosis and treatment of osteosarcoma.

Keywords: Hsa_circ_0087302, Osteosarcoma, Oncogenic circRNA, Wnt/β-catenin