Int J Med Sci 2022; 19(4):779-788. doi:10.7150/ijms.71769 This issue

Research Paper

Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury

Gang Li1,2#, Shaoping Li1#, Ruining Liu1, Jiangtao Yu1, Haoli Ma1,2✉, Yan Zhao1✉

1. Emergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
2. Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
#These authors contributed equally to this work.

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Citation:
Li G, Li S, Liu R, Yu J, Ma H, Zhao Y. Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury. Int J Med Sci 2022; 19(4):779-788. doi:10.7150/ijms.71769. Available from https://www.medsci.org/v19p0779.htm

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Abstract

Graphic abstract

Traumatic brain injury is a medical event of global concern, and a growing body of research suggests that circular RNAs can play very important roles in traumatic brain injury. To explore the functions of more novel and valuable circular RNA in traumatic brain injury response, a moderate traumatic brain injury in rats was established and comprehensive analysis of circular RNA expression profiles in rat cerebral cortex was done. As a result, 301 up-regulated and 284 down-regulated circular RNAs were obtained in moderate traumatic brain injury rats, the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed based on the circular RNA's host genes, and a circRNA-miRNA interaction network based on differentially expressed circular RNAs was constructed. Also, four circular RNAs were validated by RT-qPCR and Sanger sequencing. This study showed that differentially expressed circular RNAs existed between rat cerebral cortex after moderate traumatic brain injury and control. And this will provide valuable information for circular RNA research in the field of traumatic brain injury.

Keywords: circular RNA, moderate traumatic brain injury, brain injury, gene expression profiles, rat