Int J Med Sci 2021; 18(8):1877-1885. doi:10.7150/ijms.52177 This issue

Research Paper

Hydroxyurea regulates the development and survival of B16 Melanoma Cells by upregulating MiR-7013-3p

Yi-Jie Huang, Yan Gao, Chang-Jiang Wang, Dong-Xu Han, Yi Zheng, Wen-Hua Wang, Hao Jiang, Bao Yuan, Jia-Bao Zhang

Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, Jilin, P.R. China.

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Citation:
Huang YJ, Gao Y, Wang CJ, Han DX, Zheng Y, Wang WH, Jiang H, Yuan B, Zhang JB. Hydroxyurea regulates the development and survival of B16 Melanoma Cells by upregulating MiR-7013-3p. Int J Med Sci 2021; 18(8):1877-1885. doi:10.7150/ijms.52177. Available from https://www.medsci.org/v18p1877.htm

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Abstract

Graphic abstract

miRNAs are a family of short, noncoding RNAs that are involved in many processes in melanoma cells. MITF acts as a master regulator of melanocyte function, development and survival by modulating various genes. Hydroxyurea (HU) is used to treat melanoma, and miRNA expression is altered after HU treatment in B16 melanoma cells. In this study, we screened for miRNAs that were upregulated after HU treatment and that targeted the MITF gene. We found that miR-7013-3p exhibited increased expression after HU treatment and could bind to MITF. miR-7013-3p inhibited melanin production, proliferation, and migration and promoted apoptosis in B16 melanoma cells. The results may provide more information on the roles of miR-7013-3p in B16 melanoma cells.

Keywords: miR-7013-3p, B16 melanoma cells, MITF, hydroxyurea (HU), pigmentation