Int J Med Sci
2020; 17(18):3065-3072.
doi:10.7150/ijms.49533 This issueCite
Research Paper
IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway
Zongtao Liu*, Yixuan Wang*, Jiawei Shi, Si Chen, Li Xu, Fei Li✉, Nianguo Dong✉
Department of Cardiovascular Surgery, Wuhan Union Hospital, Huazhong University of Science and Technology, Wuhan, China. *These authors contributed equally to this work.
✉ Corresponding authors: Dr. Nianguo Dong, MD, PhD, Union Hospital, Wuhan Jiefang Road, No. 1277, Wuhan 430022, China. Telephone: +86-139-7118-1551. E-mail: dongnianguocom. Dr. Fei Li, MD, Union Hospital, Wuhan Jiefang Road, No. 1277, Wuhan 430022, China. Telephone: +86-134-3713-8546. E-mail: lifei_unioncom.More
Citation:
Liu Z, Wang Y, Shi J, Chen S, Xu L, Li F, Dong N. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 2020; 17(18):3065-3072. doi:10.7150/ijms.49533. https://www.medsci.org/v17p3065.htm
Objectives: This study amied to whether IL-21 promotes osteoblast transdifferentiation of cultured human Valvular interstitial cells (VICs).
Methods: We first confirmed that IL-21 alters gene expression between CAVD aortic valve tissue and normal samples by immunohistochemistry, qPCR, and western blotting. VICs were cultured and treated with IL-21. Gene and protein expression levels of the osteoblastic markers ALP and Runx2, which can be blocked by specific JAK3 inhibitors and/or siRNA of STAT3, were measured.
Results: IL-21 expression was upregulated in calcified aortic valves and promotes osteogenic differentiation of human VICs. IL-21 accelerated VIC calcification through the JAK3/STAT3 pathway.
Conclusion: Our data suggest that IL-21 is a key factor in valve calcification and a promising candidate for targeted therapeutics for CAVD.
Liu, Z., Wang, Y., Shi, J., Chen, S., Xu, L., Li, F., Dong, N. (2020). IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. International Journal of Medical Sciences, 17(18), 3065-3072. https://doi.org/10.7150/ijms.49533.
ACS
Liu, Z.; Wang, Y.; Shi, J.; Chen, S.; Xu, L.; Li, F.; Dong, N. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int. J. Med. Sci. 2020, 17 (18), 3065-3072. DOI: 10.7150/ijms.49533.
NLM
Liu Z, Wang Y, Shi J, Chen S, Xu L, Li F, Dong N. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 2020; 17(18):3065-3072. doi:10.7150/ijms.49533. https://www.medsci.org/v17p3065.htm
CSE
Liu Z, Wang Y, Shi J, Chen S, Xu L, Li F, Dong N. 2020. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci. 17(18):3065-3072.
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