Int J Med Sci 2020; 17(15):2312-2327. doi:10.7150/ijms.47766 This issue Cite

Research Paper

Characterization and functional prediction of the microRNAs differentially expressed in a mouse model of concanavalin A-induced autoimmune hepatitis

Yang Liu1,2, Hao Chen1,2, Jianheng Hao1,2, Zhencheng Li1,2, Tiezheng Hou1,2✉#, Huiqin Hao1,2✉#

1. College of Basic Medical Sciences, Shanxi University of Chinese Medicine, Jinzhong, 030619, PR China.
2. Basic Laboratory of Integrated Traditional Chinese and Western Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, PR China.
#Co-senior authors with equal contributions to this work.

Citation:
Liu Y, Chen H, Hao J, Li Z, Hou T, Hao H. Characterization and functional prediction of the microRNAs differentially expressed in a mouse model of concanavalin A-induced autoimmune hepatitis. Int J Med Sci 2020; 17(15):2312-2327. doi:10.7150/ijms.47766. https://www.medsci.org/v17p2312.htm
Other styles

File import instruction

Abstract

In order to investigate the altered expression of microRNAs (miRNAs) in the development of autoimmune hepatitis (AIH), the aberrantly expressed miRNAs in the concanavalin A (Con A)-induced AIH mouse model were identified for the first time with microarray in this study. A total of 49 miRNAs (31 up- and 18 down-regulated) were screened out, and the qRT-PCR validation results of 12 chosen miRNAs were consistent with the microarray data. Combined with the profiling of differently expressed mRNAs in the same model (data not shown), 959 predicted target genes (601 for up- and 358 for down-regulated miRNAs) were obtained according to the intersection of databases miRWalk and miRDB, and several hub genes were obtained from the regulatory networks, including Cadm1 and Mier3. These target genes were significantly enriched in the Gene ontology (GO) terms of “transcription, DNA-templated”, and were annotated in 47 signaling pathways, comprising “Wnt signaling pathway”, “Hippo signaling pathway”, “Ferroptosis” and “mitogen-activated protein kinase (MAPK) signaling pathway”, according to the GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In the miRNA-GO-network, mmu-miR-193b-3p were exhibited in 33 GO terms of biological processes (BP), and the most significantly regulated GO term in BP categories was “regulation of transcription, DNA-templated”. While in the miRNA-pathway-network, mmu-miR-7005-5p were enriched in 37 pathways, which was more than the other specifically expressed miRNAs, and the most significantly enriched pathways were “Endocytosis” and “MAPK signaling pathway”. In conclusion, these differently expressed miRNAs seemed to be associated with the onset of AIH, and have the potential to serve as the new targets on the treatment of this disease.

Keywords: autoimmune hepatitis, concanavalin A, microRNA, microarray, Gene Ontology, KEGG


Citation styles

APA
Liu, Y., Chen, H., Hao, J., Li, Z., Hou, T., Hao, H. (2020). Characterization and functional prediction of the microRNAs differentially expressed in a mouse model of concanavalin A-induced autoimmune hepatitis. International Journal of Medical Sciences, 17(15), 2312-2327. https://doi.org/10.7150/ijms.47766.

ACS
Liu, Y.; Chen, H.; Hao, J.; Li, Z.; Hou, T.; Hao, H. Characterization and functional prediction of the microRNAs differentially expressed in a mouse model of concanavalin A-induced autoimmune hepatitis. Int. J. Med. Sci. 2020, 17 (15), 2312-2327. DOI: 10.7150/ijms.47766.

NLM
Liu Y, Chen H, Hao J, Li Z, Hou T, Hao H. Characterization and functional prediction of the microRNAs differentially expressed in a mouse model of concanavalin A-induced autoimmune hepatitis. Int J Med Sci 2020; 17(15):2312-2327. doi:10.7150/ijms.47766. https://www.medsci.org/v17p2312.htm

CSE
Liu Y, Chen H, Hao J, Li Z, Hou T, Hao H. 2020. Characterization and functional prediction of the microRNAs differentially expressed in a mouse model of concanavalin A-induced autoimmune hepatitis. Int J Med Sci. 17(15):2312-2327.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image