Int J Med Sci 2020; 17(4):428-436. doi:10.7150/ijms.41899

Research Paper

The effect of repetitive exposure to intravenous anesthetic agents on the immunity in mice

Hyun Jun Park1, Liyun Piao1, Eun-Hye Seo2, Seung Hyun Lee3,4*, Seong-Hyop Kim1,4,5*✉

1. Department of Infection and Immunology, Konkuk University School of Medicine, Seoul, Korea.
2. BK21 plus, Department of Cellular and Molecular Medicine, Konkuk University School of Medicine, Seoul, Korea.
3. Department of Microbiology, Konkuk University School of Medicine, Seoul, Korea.
4. Department of Medicine, Institute of Biomedical Science and Technology, Konkuk University School of Medicine, Seoul, Korea.
5. Department of Anesthesiology and Pain medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea.
*The two authors evenly contributed to the study as corresponding author.

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Citation:
Park HJ, Piao L, Seo EH, Lee SH, Kim SH. The effect of repetitive exposure to intravenous anesthetic agents on the immunity in mice. Int J Med Sci 2020; 17(4):428-436. doi:10.7150/ijms.41899. Available from http://www.medsci.org/v17p0428.htm

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Abstract

Introduction: This study was designed to assess the effect of repetitive exposure to intravenous anesthetic agents on the immunity in mice.

Materials and Methods: The mice were divided into six groups: three intravenous anesthetic agents groups (dexmedetomidine, midazolam and propofol groups), and three corresponding control groups (CD, CM, and CP groups). The intravenous injections were administered once per day for 5 days. The immunity of mice was checked after the last intravenous injection. Histopathology and immunochemistry of liver and kidneys were evaluated. Cytokine levels in the blood was also checked. vs. evaluated with cytokine levels in the blood.

Results: Cluster of differentiation (CD)4+ T cells were significantly less expressed in dexmedetomidine and propofol groups, compared with the corresponding control groups [34.08 ± 5.63% in the dexmedetomidine group vs. 59.74 ± 8.64% in the CD group, p < 0.05; 25.28 ± 7.28% in the propofol group vs. 61.12 ± 2.70% in the Cp group, p < 0.05]. Apoptosis of CD4+ T cells was increased significantly in dexmedetomidine and propofol groups, compared with the corresponding control groups. Histopathological findings of liver and kidneys did not show any specific differences of any of three intravenous anesthetic agents groups with their corresponding control groups, although immunohistochemical examination indicated significantly lower expression of Toll-like receptor-4 from liver and kidneys in dexmedetomidine and propofol groups. The cytokine levels were not different between the groups.

Conclusion: Repetitive exposure to dexmedetomidine and propofol reduced the expression of CD4+ T cells but did not induce any significant liver or kidney injuries.

Keywords: intravenous anesthetic agent, dexmedetomidine, midazolam, propofol, immunity