Int J Med Sci 2019; 16(12):1557-1563. doi:10.7150/ijms.39068

Research Paper

Critical Roles of E2F3 in Growth and Musculo-skeletal Phenotype in Mice

Hae-Rim Kim1*, Faiz Ur Rahman1*, Kwang-Soo Kim1,2*, Eun-Kyeung Kim1, Sang-Mi Cho1, Kihoon Lee1, Ok-sung Moon1, Young-won Seo1, Won-Kee Yoon1, Young-Suk Won1, Hoyoung Kang1, Hyoung-Chin Kim1, Ki-Hoan Nam1✉

1. Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yeonjudanji-ro 30, Chungbuk 28116, Korea
2. Department of Animal Science and Technology, Chung-Ang University, Seodong-daero 4726, Gyeonggi 17546, Korea
*These authors are contributed equally to this study.

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Citation:
Kim HR, Rahman FU, Kim KS, Kim EK, Cho SM, Lee K, Moon Os, Seo Yw, Yoon WK, Won YS, Kang H, Kim HC, Nam KH. Critical Roles of E2F3 in Growth and Musculo-skeletal Phenotype in Mice. Int J Med Sci 2019; 16(12):1557-1563. doi:10.7150/ijms.39068. Available from http://www.medsci.org/v16p1557.htm

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Abstract

E2F3, a member of the E2F family, plays a critical role in cell cycle and proliferation by targeting downstream, retinoblastoma (RB) a tumor suppressor family protein. The purpose of this study, was to investigate the role and function of E2F3 in vivo. We examined phenotypic abnormalities, by deletion of the E2f3 gene in mice. Complete ablation of the E2F3 was fully penetrant, in the pure C57BL/6N background. The E2f3+/- mouse embryo developed normally without fatal disorder. However, they exhibited reduced body weight, growth retardation, skeletal imperfection, and poor grip strength ability. Findings suggest that E2F3 has a pivotal role in muscle and bone development, and affect normal mouse growth.

Keywords: E2f3+/- Knockout, Development, Growth retardation, skeletal imperfection, Mouse phenotype