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Int J Med Sci 2014; 11(11):1116-1128. doi:10.7150/ijms.9611 This issue Cite

Research Paper

Cultured Human Periosteal-Derived Cells Have Inducible Adipogenic Activity and Can Also Differentiate Into Osteoblasts in a Perioxisome Proliferator-Activated Receptor-Mediated Fashion

Young-Sool Hah1*, Hyun-Ho Joo2*, Young-Hoon Kang2, Bong-Wook Park2, Sun-Chul Hwang3, Jong-Woo Kim4, Iel-Yong Sung5, Gyu-Jin Rho6, Dong Kyun Woo7✉, June-Ho Byun2✉

1. Clinical Research Institute of Gyeongsang National University Hospital, Jinju, Republic of Korea;
2. Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Republic of Korea;
3. Department of Orthopaedic Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Republic of Korea;
4. Department of Thoracic and Cardiovascular Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Republic of Korea;
5. Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University, Ulsan, Republic of Korea;
6. OBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea;
7. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, Republic of Korea.
* These authors contributed equally to this work.

✉ Corresponding authors: June-Ho Byun (Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Biomedical center (BK21), 660-702, Gyeongsang National University School of Medicine, Jinju, Republic of Korea, Tel: 82-55-750-8258, Fax More

Citation:
Hah YS, Joo HH, Kang YH, Park BW, Hwang SC, Kim JW, Sung IY, Rho GJ, Woo DK, Byun JH. Cultured Human Periosteal-Derived Cells Have Inducible Adipogenic Activity and Can Also Differentiate Into Osteoblasts in a Perioxisome Proliferator-Activated Receptor-Mediated Fashion. Int J Med Sci 2014; 11(11):1116-1128. doi:10.7150/ijms.9611. https://www.medsci.org/v11p1116.htm
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Abstract

We investigated the adipogenic activity of cultured human periosteal-derived cells and studied perioxisome proliferator-activated receptor (PPAR) ligand-mediated differentiation of cultured human periosteal-derived cells into osteoblasts. Periosteal-derived cells expressed adipogenic markers, including CCAAT/enhancer binding protein α (C/EBP- α), C/EBP-δ, aP2, leptin, LPL, and PPARγ. Lipid vesicles were formed in the cytoplasm of periosteal-derived cells. Thus, periosteal-derived cells have potential adipogenic activity. The PPARα and PPARγ agonists, WY14643 and pioglitazone, respectively, did not modulate alkaline phosphatase (ALP) activity in periosteal-derived cells during induced osteoblastic differentiation, however, the PPARα and PPARγ antagonists, GW6471 and T0070907, respectively, both decreased ALP activity in these cells. WY14643 did not affect, whereas pioglitazone enhanced, alizarin red-positive mineralization and calcium content in the periosteal-derived cells. GW6471 and T0070907 both decreased mineralization and calcium content. By RT-PCR, pioglitazone significantly increased ALP expression in periosteal-derived cells between culture day 3 and 2 weeks. Pioglitazone increased Runx2 expression after 3 days, which declined thereafter, but did not alter osteocalcin expression. Both of GW6471 and T0070907 decreased ALP mRNA expression. These results suggest that pioglitazone enhances osteoblastic differentiation of periosteal-derived cells by increasing Runx2 and ALP mRNA expression, and increasing mineralization. GW6471 and T0070907 inhibit osteoblastic differentiation of the periosteal-derived cells by decreasing ALP expression and mineralization in the periosteal-derived cells.

In conclusion, although further study will be needed to clarify the mechanisms of PPAR-regulated osteogenesis, our results suggest that PPARγ agonist stimulates osteoblastic differentiation of cultured human periosteal-derived cells and PPARα and PPARγ antagonists inhibit osteoblastic differentiation in these cells.

Keywords: Periosteal-derived cells, Adipogenic activity, Osteoblastic differentiation, PPAR ligands.

Citation styles

APA
Hah, Y.S., Joo, H.H., Kang, Y.H., Park, B.W., Hwang, S.C., Kim, J.W., Sung, I.Y., Rho, G.J., Woo, D.K., Byun, J.H. (2014). Cultured Human Periosteal-Derived Cells Have Inducible Adipogenic Activity and Can Also Differentiate Into Osteoblasts in a Perioxisome Proliferator-Activated Receptor-Mediated Fashion. International Journal of Medical Sciences, 11(11), 1116-1128. https://doi.org/10.7150/ijms.9611.

ACS
Hah, Y.S.; Joo, H.H.; Kang, Y.H.; Park, B.W.; Hwang, S.C.; Kim, J.W.; Sung, I.Y.; Rho, G.J.; Woo, D.K.; Byun, J.H. Cultured Human Periosteal-Derived Cells Have Inducible Adipogenic Activity and Can Also Differentiate Into Osteoblasts in a Perioxisome Proliferator-Activated Receptor-Mediated Fashion. Int. J. Med. Sci. 2014, 11 (11), 1116-1128. DOI: 10.7150/ijms.9611.

NLM
Hah YS, Joo HH, Kang YH, Park BW, Hwang SC, Kim JW, Sung IY, Rho GJ, Woo DK, Byun JH. Cultured Human Periosteal-Derived Cells Have Inducible Adipogenic Activity and Can Also Differentiate Into Osteoblasts in a Perioxisome Proliferator-Activated Receptor-Mediated Fashion. Int J Med Sci 2014; 11(11):1116-1128. doi:10.7150/ijms.9611. https://www.medsci.org/v11p1116.htm

CSE
Hah YS, Joo HH, Kang YH, Park BW, Hwang SC, Kim JW, Sung IY, Rho GJ, Woo DK, Byun JH. 2014. Cultured Human Periosteal-Derived Cells Have Inducible Adipogenic Activity and Can Also Differentiate Into Osteoblasts in a Perioxisome Proliferator-Activated Receptor-Mediated Fashion. Int J Med Sci. 11(11):1116-1128.

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