Int J Med Sci 2013; 10(10):1406-1411. doi:10.7150/ijms.6565

Research Paper

Identification and Determination of Antibiotic Susceptibilities of Brucella Strains Isolated from Patients in Van, Turkey by Conventional and Molecular Methods

Mehmet Parlak1, Hüseyin Güdücüoğlu2, Yasemin Bayram2, Aytekin Çıkman3, Cenk Aypak4✉, Selçuk Kılıç5, Mustafa Berktaş2

1. Microbiology Laboratory, Van Education and Training Hospital, Van, Turkey;
2. Department of Medical Microbiology, Yuzuncu Yil University, Faculty of Medicine, Van, Turkey;
3. Microbiology Laboratory, Mengucek Gazi Education and Training Hospital, Erzincan, Turkey;
4. Department of Family Medicine, Diskapi Yildirim Beyazit Education and Training Hospital, Ankara, Turkey;
5. Bacterial Zoonoses Research Reference Laboratory, Turkey Public Health Agency, Ankara, Turkey.

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Parlak M, Güdücüoğlu H, Bayram Y, Çıkman A, Aypak C, Kılıç S, Berktaş M. Identification and Determination of Antibiotic Susceptibilities of Brucella Strains Isolated from Patients in Van, Turkey by Conventional and Molecular Methods. Int J Med Sci 2013; 10(10):1406-1411. doi:10.7150/ijms.6565. Available from

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Purpose: Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In this study, we aimed to identify biovars of Brucella strains isolated from clinical specimens taken from brucellosis patients from the Eastern Anatolia region as well determine the susceptibility of these isolates to tigecycline and azithromycin, drugs that may serve as alternatives to the conventional drugs used in the therapy.

Materials and methods: Seventy-five Brucella spp. isolates were included in the study. All strains were identified by both conventional and molecular methods. Brucella Multiplex PCR kit (FC-Biotech, Code: 0301, Turkey) and B. melitensis biovar typing PCR kit (FC-Biotech, Code: 0302, Turkey) were used for molecular typing. Antimicrobial susceptibilities of all strains were determined by E-tests.

Results: By conventional biotyping, 73 strains were identified as B. melitensis biovar 3 and two strains as B. abortus biovar 3. Molecular typing results were compatible with conventional methods. The MIC50 and MIC90 values of doxycycline were 0.047 and 0.094; tigecycline 0.094 and 0.125; trimethoprim/sulfamethoxazole 0.064 and 0.19; ciprofloxacin 0.19 for both; streptomycin 0.75 and 1; rifampin 1 and 2 and azithromycin 4 and 8. According to the MIC values, doxycycline was found to be the most effective antibiotic, followed by tigecycline, trimethoprim-sulfamethoxazole and ciprofloxacin.

Conclusion: Currently recommended antibiotics for the treatment of brucellosis such as doxycycline, rifampin, streptomycin, trimethoprim-sulfamethoxazole and ciprofloxacin were found to be still effective. While our results showed that tigecycline can be used an alternative agent in the treatment of brucellosis, azithromycin has not been confirmed as an appropriate agent for the treatment.

Keywords: Brucella, biotyping, antibiotic sensitivity, E-test.