Int J Med Sci 2012; 9(10):862-871. doi:10.7150/ijms.5027
Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner
1. Department of Stomatology, Research Center for Oral Diseases and Biomedicine in Chongqing, Affiliated Stomatological Hospital, Chongqing Medical University, Chongqing 400015, China;
2. Key Laboratory of Clinical Laboratory Science of the Ministry of Education, Chongqing Medical University, China.
Li C, Yang X, He Y, Ye G, Li X, Zhang X, Zhou L, Deng F. Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner. Int J Med Sci 2012; 9(10):862-871. doi:10.7150/ijms.5027. Available from http://www.medsci.org/v09p0862.htm
Dental follicle stem cells are a group of cells possessing osteogenic, adipogenetic and neurogenic differentiations, but the specific mechanism underlying the multilineage differentiation remains still unclear. Great attention has been paid to bone morphogenetic protein-9 (BMP-9) due to its potent osteogenic activity. In the present study, rat dental follicle stem cells were isolated and purified, and cells of passage 3 underwent adenovirus mediated BMP-9 gene transfection to prepare dental follicle stem cells with stable BMP-9 expression. Detection of alkaline phosphatase (ALP) and calcium deposition showed dental follicle stem cells transfected with BMP-9 gene could significantly promote the osteogenesis. In addition, SB203580 and PD98059 were employed to block the p38 mitogen-activated protein kinase (p38MAPK) and extracellular signal-regulated kinase (ERK1/2), respectively. Detection of ALP and calcium deposition revealed the BMP-9 induced osteogenic differentiation of dental follicle stem cells depended on MAPK signaling pathway.
Keywords: Dental follicle stem cells, rat recombinant adenovirus transfection, bone morphogenetic protein-9, osteogenic differentiation, signaling pathway.