International Journal of Medical Sciences

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15 December 2017

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Int J Med Sci 2017; 14(12):1276-1283. doi:10.7150/ijms.20479

Research Paper

Cell Recognition Molecule L1 Regulates Cell Surface Glycosylation to Modulate Cell Survival and Migration

Gang Shi1✉, Yue Du2, Yali Li3✉, Yue An4, Zhenwei He5, Yingwei Lin4, Rui Zhang1, Xiaofei Yan1, Jianfeng Zhao1, Shihua Yang1, Pang Nghee Kheem Brendan3, Fang Liu1✉

1. Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, China;
2. Dalian Medical University, Dalian, Liaoning 116044, China;
3. National University Hospital, Singapore 119074, Singapore;
4. Department of Clinical Laboratory, the Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, China;
5. Department of Neurology, Forth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110000, China.

Abstract

Background: Cell recognition molecule L1 (L1) plays an important role in cancer cell differentiation, proliferation, migration and survival, but its mechanism remains unclear.

Methodology/Principal: Our previous study has demonstrated that L1 enhanced cell survival and migration in neural cells by regulating cell surface glycosylation. In the present study, we show that L1 affected cell migration and survival in CHO (Chinese hamster ovary) cell line by modulation of sialylation and fucosylation at the cell surface via the PI3K (phosphoinositide 3-kinase) and Erk (extracellularsignal-regulated kinase) signaling pathways. Flow cytometry analysis indicated that L1 modulated cell surface sialylation and fucosylation in CHO cells. Activated L1 upregulated the protein expressions of ST6Gal1 (β-galactoside α-2,6-sialyltransferase 1) and FUT9 (Fucosyltransferase 9) in CHO cells. Furthermore, activated L1 promoted CHO cells migration and survival as shown by transwell assay and MTT assay. Inhibitors of sialylation and fucosylation blocked L1-induced cell migration and survival, while decreasing FUT9 and ST6Gal1 expressions via the PI3K-dependent and Erk-dependent signaling pathways.

Conclusion: L1 modulated cell migration and survival by regulation of cell surface sialylation and fucosylation via the PI3K-dependent and Erk-dependent signaling pathways.

Keywords: Cell adhesion molecule L1, Glycosylation, Sialylation, Fucosylation, CHO cells.

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How to cite this article:
Shi G, Du Y, Li Y, An Y, He Z, Lin Y, Zhang R, Yan X, Zhao J, Yang S, Brendan PNK, Liu F. Cell Recognition Molecule L1 Regulates Cell Surface Glycosylation to Modulate Cell Survival and Migration. Int J Med Sci 2017; 14(12):1276-1283. doi:10.7150/ijms.20479. Available from http://www.medsci.org/v14p1276.htm