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25 May 2019

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Int J Med Sci 2017; 14(12):1263-1267. doi:10.7150/ijms.21023

Research Paper

Overexpression of Endothelial Cell-Specific Molecule 1 Correlates with Gleason Score and Expression of Androgen Receptor in Prostate Carcinoma

Chung-Yu Lai1, 2, 3, Chien-Min Chen4, 5, Wen-Hung Hsu6, Yi-Hsien Hsieh7, 8* Corresponding address, Chung-Jung Liu6, 9* Corresponding address

1. Department of Surgery, Chung-Kang Branch, Cheng-Ching General Hospital, Taichung, Taiwan;
2. Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan;
3. Center for General Education, School of Medicine, Chung Shan Medical University, Taichung, Taiwan;
4. Division of Neurosurgery, Department of Surgery, Changhua Christian Hospital, Changhua, Taiwan;
5. School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;
6. Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;
7. Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan;
8. Clinical laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan;
9. Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan.
* These authors contributed equally

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license ( See for full terms and conditions.
How to cite this article:
Lai CY, Chen CM, Hsu WH, Hsieh YH, Liu CJ. Overexpression of Endothelial Cell-Specific Molecule 1 Correlates with Gleason Score and Expression of Androgen Receptor in Prostate Carcinoma. Int J Med Sci 2017; 14(12):1263-1267. doi:10.7150/ijms.21023. Available from


Endothelial cell-specific molecule 1 (ESM1) is a major prognostic marker of several tumor types, but its value as a marker for prostate cancer is unknown. The purpose of the present study was to measure the relationship of ESM1 expression with androgen receptor (AR) expression and with Gleason score in human prostate carcinoma tissue. Expression of ESM1 and AR were determined by immunohistochemical staining of prostate tissues from healthy individuals and patients with prostate cancer. The results showed that ESM1 expression was significantly higher in prostate tumor tissues than in normal prostate tissues (p < 0.01), and that ESM1 expression in prostate tumor tissue correlated with Gleason score (p < 0.016) and Gleason grade (p < 0.013). ESM1 expression was also greater in prostate tissues with higher Gleason score and Gleason grade (p < 0.001 for both comparisons), and also correlated with AR expression (R = 0.727, p < 0.001). In conclusion, our results demonstrated that ESM1 should be considered as a marker for the diagnosis of prostate cancer.

Keywords: Human prostate cancer, Gleason score, Endothelial cell specific molecule 1, Androgen Receptor.


Prostate carcinoma (PC) is one of the most prevalent cancers world-wide, and is the second-most diagnosed cancer in men [1]. Certain dietary factors, lifestyle factors, and prostate cancer-susceptibility alleles, such as specific alleles of the androgen receptor (AR), increase the risk for development of PC [2]. The Gleason score of PC is a well-established prognostic indicator [3], and clinicians often use the Gleason score to estimate the response to a specific therapy, such as radiotherapy and/or surgery. The discovery of additional biomarkers by gene expression profiling or testing for DNA abnormalities may help to better predict prognosis and provide value beyond the Gleason score [3].

Endothelial cell-specific molecule 1 (ESM1), also known as endocan, is secreted into the blood as a 50 kDa soluble proteoglycan that has 165 amino acids as a mature polypeptide. Lassalle and colleagues originally cloned ESM1 from a human umbilical vein endothelial cell (HUVEC) cDNA library [4]. In normal human tissues, ESM1 is selectively expressed in actively proliferative and neogeneic tissues and cells, such as glandular tissues, endothelial cells of neovasculature tissues, the bronchial epithelium, and germinal centers of lymph nodes [5]. Inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), unregulated ESM1 mRNA, and this upregulation is associated with tumor growth and angiogenesis during tumor progression [4, 6, 7]. ESM1 also promotes tumor growth in a mouse model with human tumor xenografts [7], and its expression correlates with the development of lung, renal, and breast cancers [8-11].

More generally, ESM1 is regarded as a marker of angiogenesis. ESM1-mediated promotion of microvessel density correlates with microscopic venous invasion and expression of vascular endothelial growth factor (VEGF) in patients with hepatocellular carcinoma [12]. The circulating level of ESM1 is also markedly increased in the serum of patients with advanced lung cancer [7], and ESM1 expression is associated with tumor prognosis, metastasis, and angiogenesis. Thus, ESM1 may act as a marker for angiogenesis or oncogenesis, and could be regarded as a candidate gene for identification of inflammatory tissue, neoplasia, metastasis, and tumor development [5]. The present study examines the relationships between ESM1 expression, Gleason score, and AR expression in human prostate carcinoma tissues and prostate tissue of healthy individuals to assess its potential as a marker for diagnosis and prediction of prognosis in patients with PC.

Materials and Methods

Human prostate cancer tissues array

The tissue microarrays (TMAs) from a wide range of prostate tissues were assembled using a microarray with human prostate cancer specimens and normal prostate tissue (PR803b; US Biomax, Inc). The prostate patient data and clinicopathological characteristics, including age, sex, tumor stage, tumor grade, Gleason grade, and Gleason score, were obtained from medical records. Prostate tissues from 71 patients were examined by immunohistochemical (IHC) staining for ESM1 and AR. Digital images were acquired from 4-μm thick sections stained with hematoxylin and eosin (H&E), and from immunostained TMA slides using the Pannoramic MIDI II System (Budapest, Hungary).

Immunohistochemistry (IHC) Staining

IHC staining was performed on sections of paraffin-embedded tissues. All formalin-fixed prostate tissues were embedded in paraffin, and 5-µm sections were prepared. Sections were processed by heating at 70℃ in an oven for 30 min, dewaxing with xylene and alcohol (2 cycles, 10 min each), deparaffinization, and rehydration. Then endogenous peroxidase was blocked for 20 min in 0.3% hydrogen peroxide in water. The slides were then treated for antigen retrieval in a citrate buffer (pH 6) for 10 min at 95°C (DAKO PT Link, Glostrup, Denmark). The primary antibodies were anti-ESM1 (Abnova, H00011082-M02) and anti-AR antibody (Santa Cruz, sc-815). Samples were incubated with DAKO envision that contained horseradish peroxidase conjugated goat anti-rabbit, goat anti-mouse, or rabbit anti-goat antibodies (DAKO Cytomation, Glostrup, Denmark) for 30 min. The slides were visualized using a diaminobenzidine solution (DAB+; DAKO kit).

Immunoreactivity Scoring

The intensity of IHC staining was quantified using an immunoreactivity score (IRS). The IRS is a function of the percentage of stained cells and of the staining intensity of immunoreactive cells. The percentage score was 0 when there were no positive cells; 1 or 2 when there were 1 to 25% positive cells, 3 when there were 25 to 50% positive cells; 4 when there were 50 to 90% positive cells, and 5 when there more than 90% positive cells. The intensity score was 3 for weak cell staining; 4 for moderate cell staining; and 5 for strong cell staining. The two scores were added, so the final score ranged from 3 to 5. Median scores were used for data analysis.

Statistical Analysis

Statistical analysis was performed by using the SPSS statistical software program (standard version 18.0; SPSS,Chicago, USA). Correlation between variables was evaluated by Spearman rank correlation coefficients. p < 0.01 denotes the presence of a statistically significant difference.


Relationship of ESM1 expression with clinicopathological characteristics of prostate cancer patients

Immunohistochemical staining of prostate tissues from humans indicates that ESM1 has higher expression in cancerous than normal prostate tissues (Fig 1). Table 1 summarizes the relationships between ESM-1 expression and clinicopathological parameters of prostate cancer patients. Age and tumor stage were unrelated to ESM1 expression. However, ESM1 expression was significantly greater in the prostate tumors of patients with higher Gleason grades (p = 0.013) and Gleason scores (p = 0.016) (Fig 2, Table 1).

Correlation between levels of ESM1 and AR in prostate cancer patients

Figure 3A shows representative IHC images of ESM1 and AR expression in prostate cancer patients. Quantitation of these results shows that expression of ESM1 and AR were significantly higher in patients with Gleason scores of 5 than in those with Gleason scores of 3 or 4 (p < 0.001). Analysis of the relationship between ESM1 and AR expression in prostate cancer tissues indicated a positive correlation (p < 0.001; Fig. 3B). This, our findings indicate that ESM1 expression correlates with Gleason score, and could therefore be a useful marker for the diagnosis of prostate cancer.


The present study used IHC analysis of prostate tissue to determine the relationship of ESM1 expression with Gleason grade and score and with AR expression. The results show that ESM1 expression correlates with Gleason score and grade and with AR expression in human prostate cancer. Thus, ESM1 may be useful as a prognostic indicator, by providing value beyond the established Gleason grade and score.

 Table 1 

Correlation between ESM1 expression and clinicopathological characteristics of prostate cancer patients

CharacteristicNumber of patients (%)p Value
ESM1 staining
Total number of patients37 (52.1)34 (47.9)
Age (year)
<6715 (53.6)13(46.4)0.518
≧6722 (51.2)21 (48.8)
Tumor Stage
I+II21 (56.8)16(43.2)0.238
III+IV14 (41.2)20 (58.8)
Gleason Grade
315 (68.2)7 (31.8)0.013
414 (58.3)10 (41.7)
58 (32.0)17 (68.0)
Gleason Score
313 (68.4)6 (31.6)0.016
415 (60)10 (40)
59 (33.3)18 (66.7)
 Figure 1 

ESM1 expression in normal and cancerous of human prostate tissues. Representative IHC light microscopy images showing the expression of ESM1 in normal prostate tissues and in prostate carcinoma tissues with low and high expression of ESM1. The expression of ESM1 was quantified by a pathologist. The difference was statistically significant (p < 0.01). Scale bars = 100 μm.

Int J Med Sci Image (Click on the image to enlarge.)
 Figure 2 

Gleason score and ESM1 expression in human prostate carcinomas. ESM1 was determined by IHC staining and light microscopy. Gleason score and ESM1 expression were quantified by a pathologist. The difference was statistically significant (p < 0.01). Scale bars = 100 μm

Int J Med Sci Image (Click on the image to enlarge.)
 Figure 3 

Relationship of ESM1 expression and AR expression with Gleason score in human prostate carcinomas. (A) Human prostate carcinoma tissues were stained for ESM1 and AR by IHC and visulaized by light microscopy. Gleason score, and expression of ESM-1 and AR were determined by a pathologist. (B) Correlation between the expression of ESM1 and AR The difference was highly statistically significant (p < 0.001). Scale bars = 100 μm

Int J Med Sci Image (Click on the image to enlarge.)

The Gleason grading system is the main method used to stage prostate cancer [4]. This system mainly considers the histological pattern of cells in H&E-stained sections of prostate tissue. The strength of the Gleason grading system is that it is useful for follow-up of a large variety of patients. The Gleason system has five grades (1 to 5) based on the morphology of the most common cell type, and another five grades (1 to 5) based on the morphology of the second-most common cell type. Thus the total histological score ranges from 2 to 10. The common practice is to consider a Gleason score of 2-4 as well-differentiated carcinoma, 5-7 as moderately differentiated carcinoma, and 8-10 as poorly differentiated carcinoma. However, a Gleason score of 7 usually has characteristics of high-grade carcinoma. Our findings indicate that ESM1 expression positive correlates with Gleason score.

The AR has a critical role in regulation of the proliferation of androgen-refractory prostate cancer cells, even in the absence of androgens [13]. Many somatic alterations of the AR gene are present in human prostate carcinoma tissue, especially in patients who progress despite hormonal treatment. AR gene amplification can develop during androgen deprivation therapy, and promote tumor cell growth in the presence of low androgen concentrations [14]. Increased expression of the wild-type AR gene appears to play a key role when prostate carcinoma growth occurs in the presence of androgen deprivation therapy [15]. In the absence of AR mutations, androgen-independent prostate cancer may progress through activation of ligand-independent AR signaling pathways [16, 17]. The present study shows that the AR protein is overexpressed in human prostate cancer, and its expression correlates with Gleason score and expression of ESM1.

ESM1 is a product of endothelial cells, is highly regulated by VEGF, and its expression increases significantly during the switch from dormant to angiogenic tumors in vitro and in vivo [18-20]. In addition, a study of mouse xenograft models of breast cancer, glioblastoma, osteosarcoma, and liposarcoma reported that ESM1 expression correlates with conversion of dormant tumors to exponentially growing tumors [21]. Knockdown of ESM1 affected cell proliferation and invasion through downregulation of NF-κB, EMT and the upregulation of PTEN in human colorectal cancer [22]. Recent reports showed that deletion of ESM1 reduces NGFR induced tumor growth and metastases in oral SCC cells in vitro and in vivo, providing NGFR-enhanced metastatic capacity of oral SCC cells is dependent on ESM1 expression [23]. Large scale studies of human gene expression showed that ESM1 expression correlates with the expression of angiogenic molecules, such as VEGF, VEGFR, and c-met, in various types of cancers [20]. IHC staining indicates that human ESM1 is remarkably overexpressed in the cytoplasm of endothelial cells of tumor vessels in various human cancers, such as lung cancer [7, 18], brain cancer [19], colon cancer [24], liver cancer [12], and clear cell carcinoma of the kidney [25, 26]. A study of glioblastoma indicated that ESM1 is present in endothelial cells within and at the margins of the brain tumor, and its expression correlates with grade and abnormal neoangiogenesis in these tumors [19]. Another study reported that ESM1 is a potential biomarker in tissues from patients with renal cancer; in particular IHC staining indicated ESM1 was present in the cytoplasm of endothelial cells in clear cell renal carcinoma, and was also present in small and large intra-tumor vessels [24]. Biomarkers can contribute to the clinical diagnosis of cancers. During clinical trials, biomarkers allow stratification of patient populations according to disease progression, and the monitoring of drug safety and efficacy. IHC staining of ESM1 is a simple, inexpensive, and reliable method for the analysis of tumor tissues, and is effective in guiding the treatment of hypervascular cancers. Previous studies indicated that ESM1 could be considered as a promising biomarker to assess the prognosis of tumors in the breast [8], lung [11], brain [19], ovary [27], kidney [24, 28], and liver [12]. We found that ESM1 expression correlates with Gleason grade/score and with AR expression in prostate carcinoma tissue, so ESM1 should also be considered as a biomarker for diagnosis of prostate cancer.


AR: androgen receptor; ESM1: endothelial cell-specific molecule 1; H&E: hematoxylin and eosin; IHC: immunohistochemical; IRS: immunoreactivity score; PC: prostate cancer; TMA: Tissue microarray.


This work was supported by a grant from Chung Shan Medical University and Cheng-Ching General Hospital, Taiwan (CSMU-CCGH-104-01).

Competing Interests

The authors have declared that no competing interest exists.


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Author contact

Corresponding address Corresponding authors: Dr. Yi-Hsien Hsieh, PhD, Department of Biochemistry, School of Medicine, Chung Shan Medical University, No. 110, Section 1, Chien-Kuo N. Road, Taichung, Taiwan. Email:, Tel: +886-4-24730022 ext 11673. Dr. Chung-Jung Liu, PhD, Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-you 1st road, Kaohsiung, Taiwan, email:, Tel: +886-073121101 ext 7450

Received 2017-5-15
Accepted 2017-8-7
Published 2017-9-30