International Journal of Medical Sciences

Impact factor

23 February 2019

ISSN 1449-1907 News feeds of published articles

Manuscript login | Account

open access Global reach, higher impact

Journal of Genomics in PubMed Central. Submit manuscript now...


Journal of Cancer

International Journal of Biological Sciences

Journal of Genomics


Journal of Bone and Joint Infection (JBJI)


Journal of Biomedicine

PubMed Central Indexed in Journal Impact Factor

Int J Med Sci 2017; 14(2):150-158. doi:10.7150/ijms.17763

Research Paper

Detection of Lyme Disease Bacterium, Borrelia burgdorferi sensu lato, in Blacklegged Ticks Collected in the Grand River Valley, Ontario, Canada

John D. Scott1✉, Janet E. Foley2, John F. Anderson3, Kerry L. Clark4, Lance A. Durden5

1. Research Division, Lyme Ontario, Fergus, Ontario Canada N1M 2L7;
2. Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, USA 95616;
3. Department of Entomology and Center for Vector Ecology and Zoonotic Diseases. The Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA 06504;
4. Epidemiology & Environmental Health, Department of Public Health, University of North Florida, Jacksonville, USA 32224;
5. Department of Biology, Georgia Southern University, Statesboro, Georgia 30458, USA.


We document the presence of blacklegged ticks, Ixodes scapularis, in the Grand River valley, Centre Wellington, Ontario. Overall, 15 (36%) of 42 I. scapularis adults collected from 41 mammalian hosts (dogs, cats, humans) were positive for the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.). Using real-time PCR testing and DNA sequencing of the flagellin (fla) gene, we determined that Borrelia amplicons extracted from I. scapularis adults belonged to B. burgdorferi sensu stricto (s.s.), which is pathogenic to humans and certain domestic animals. Based on the distribution of I. scapularis adults within the river basin, it appears likely that migratory birds provide an annual influx of I. scapularis immatures during northward spring migration. Health-care providers need to be aware that local residents can present with Lyme disease symptoms anytime during the year.

Keywords: Blacklegged tick, Ixodes scapularis, Lyme disease, Borrelia burgdorferi, Infection prevalence, Grand River valley.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See for full terms and conditions.
How to cite this article:
Scott JD, Foley JE, Anderson JF, Clark KL, Durden LA. Detection of Lyme Disease Bacterium, Borrelia burgdorferi sensu lato, in Blacklegged Ticks Collected in the Grand River Valley, Ontario, Canada. Int J Med Sci 2017; 14(2):150-158. doi:10.7150/ijms.17763. Available from