16 January 2018
Int J Med Sci 2016; 13(6):457-465. doi:10.7150/ijms.15548
Differential microRNA Profiles Predict Diabetic Nephropathy Progression in Taiwan
1. Department of Endocrinology & Metabolism, Taipei City Hospital, Ren-Ai Branch, Taipei, Taiwan;
Objectives: Diabetic nephropathy (DN) is a major leading cause of kidney failure. Recent studies showed that serological microRNAs (miRs) could be utilized as biomarkers to identify disease pathogenesis; the DN-related miRs, however, remained to be explored. Methods: A prospective case-control study was conducted. The clinical significance of five potential miRs (miR-21, miR-29a, miR-29b, miR-29c and miR192) in type 2 Diabetes Mellitus (T2DM) patients who have existing diabetic retinopathy with differential Albumin:Creatinine Ratio (ACR) and estimated Glomerular Filtration Rate (eGFR) was performed using quantitative RT-PCR analysis. The subjects with diabetic retinopathy enrolled in Taipei City Hospital, Taiwan, were classified into groups of normal albuminuria (ACR<30mg/g; N=12); microalbuminuria (30mg/g<ACR<300mg/g; N=17) and overt proteinuria (ACR>300mg/g; N=21) as well as 18 low-eGFR (eGFR<60ml/min) and 32 high-eGFR (eGFR>60ml/min). The level of serum miRs was statistically correlated with age, Glucose AC, ACR, eGFR and DN progression. Results: The levels of miR-21, miR-29a and miR-192 were significantly enriched in the overt proteinuria group compared with microalbuminuria and/or overt proteinuria groups. It was shown that only miR-21 level was significantly up-regulated in low-eGFR group compared with high-eGFR patients. Interestingly, Pearson's correlation coefficient analysis demonstrated that DN progressors showed significantly greater levels of miR-21, miR-29a, miR-29b and miR-29c in comparison with non-progressors implying the clinical potential of DN associated miRs in monitoring and preventing disease advancement. Conclusion: Our findings showed that miR-21, miR-29a/b/c and miR-192 could reflect DN pathogenesis and serve as biomarkers during DN progression.
Keywords: Albumin:creatinine ratio, Biomarkers, Circulating microRNA, Diabetic nephropathy, Estimated Glomerular Filtration Rate.
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How to cite this article:
Chien HY, Chen CY, Chiu YH, Lin YC, Li WC. Differential microRNA Profiles Predict Diabetic Nephropathy Progression in Taiwan. Int J Med Sci 2016; 13(6):457-465. doi:10.7150/ijms.15548. Available from http://www.medsci.org/v13p0457.htm