International Journal of Medical Sciences

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19 October 2017

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Int J Med Sci 2015; 12(1):1-6. doi:10.7150/ijms.8870

Research Paper

High Reproducibility of Adhesion Formation in Rat with Meso-Stitch Approximation of Injured Cecum and Abdominal Wall

Daniel Poehnert1✉, Mahmoud Abbas2, Hans-Heinrich Kreipe2, Juergen Klempnauer1, Markus Winny1

1. Department of General, Visceral and Transplantation Surgery, Hannover Medical School (MHH), Hannover, Germany
2. Department of Pathology, Hannover Medical School (MHH), Hannover, Germany

Abstract

Objective: Peritoneal adhesions following surgery are a common, serious pathology with severe complications. Appropriate animal adhesion models are essential for the assessment of adhesion preventing medical devices. This study introduces a variation of an established rat model in which highest degree adhesions are induced with excellent reproducibility (OPAM = optimized peritoneal adhesion model). Thus, this model seems to be eligible to study effects of adhesion preventing devices.

Methods: 24 Lewis male rats were divided into four groups (OPAM, WSFX, sham-OPAM, sham-WSFX). The OPAM technique comprised cecal abrasion, creation of an abdominal wall defect plus approximation of injured areas by a suture, which was compared to a setting of lesions without suture fixation (WSFX). All rats were sacrificed at day 7. Macroscopic and histopathological evaluations were performed. Results were statistically analyzed using ANOVA and Dunnett's test.

Results: In OPAM rats macroscopic analyses revealed a 90% incidence adhesion of cecum to the abdominal wall, all adhesions imposing as complete agglutination. In WSFX animals incidence of adhesions formation was 75%, while in both sham groups there were no adhesions at all. Histology showed the structure of adhesions with merged smooth muscle of colon and skeletal muscle of abdominal wall in all cases.

Conclusion: OPAM technique provides adhesions of injured areas with a better probability than with conventional methods. All OPAM adhesions impressed as highest degree adhesions, i.e. agglutination. Due to high reproducibility in incidence and extend of adhesion formation, the OPAM is recommended for testing of adhesion prevention medical devices.

Keywords: Adhesion, Prevention, Rat, Cecal Abrasion, OPAM.

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How to cite this article:
Poehnert D, Abbas M, Kreipe HH, Klempnauer J, Winny M. High Reproducibility of Adhesion Formation in Rat with Meso-Stitch Approximation of Injured Cecum and Abdominal Wall. Int J Med Sci 2015; 12(1):1-6. doi:10.7150/ijms.8870. Available from http://www.medsci.org/v12p0001.htm