Zheng C, Baum BJ. All Human EF1α Promoters Are Not Equal: Markedly Affect Gene Expression in Constructs from Different Sources. Int J Med Sci 2014; 11(5):404-408. doi:10.7150/ijms.8033. https://www.medsci.org/v11p0404.htm
The promoter is a major element in the expression cassette of gene therapy vectors. Optimal promoter selection can enhance target specificity and gene expression. Recently, we evaluated three different human elongation factor 1 alpha (EF1α) promoters. The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA. The activity from one EF1α promoter (termed EF1α -3), obtained in a commercial vector, was markedly lower when tested in vitro (from 50 - 500 x) in four cell lines and in vivo in rat submandibular glands (~250 x). Sequence differences in the EF1α -3 promoter likely account for the activity differences seen. Investigators need to recognize that all promoters of the same name may not be equivalent in driving transgene expression.
Keywords: promoter, EF1α, gene therapy.
Citation styles
APA
Zheng, C., Baum, B.J. (2014). All Human EF1α Promoters Are Not Equal: Markedly Affect Gene Expression in Constructs from Different Sources. International Journal of Medical Sciences, 11(5), 404-408. https://doi.org/10.7150/ijms.8033.
ACS
Zheng, C.; Baum, B.J. All Human EF1α Promoters Are Not Equal: Markedly Affect Gene Expression in Constructs from Different Sources. Int. J. Med. Sci. 2014, 11 (5), 404-408. DOI: 10.7150/ijms.8033.
NLM
Zheng C, Baum BJ. All Human EF1α Promoters Are Not Equal: Markedly Affect Gene Expression in Constructs from Different Sources. Int J Med Sci 2014; 11(5):404-408. doi:10.7150/ijms.8033. https://www.medsci.org/v11p0404.htm
CSE
Zheng C, Baum BJ. 2014. All Human EF1α Promoters Are Not Equal: Markedly Affect Gene Expression in Constructs from Different Sources. Int J Med Sci. 11(5):404-408.