International Journal of Medical Sciences

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22 October 2017

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Int J Med Sci 2014; 11(3):298-308. doi:10.7150/ijms.6672

Research Paper

Effects of ECM Protein Mimetics on Adhesion and Proliferation of Chorion Derived Mesenchymal Stem Cells

Ji-Hyun Kim1,3, Dong Wook Jekarl1, Myungshin Kim1, Eun-Jee Oh1, Yonggoo Kim1✉, In Yang Park2, Jong Chul Shin2

1. Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, South Korea;
2. Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, South Korea;
3. Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Seoul, South Korea.

Abstract

Background: We evaluated the effects of fibronectin, collagen, cadherin, and laminin based extracellular matrix (ECM) protein mimetics coated with mussel derived adhesive protein (MAP) on adhesion and proliferation of chorionic mesenchymal stem cells (cMSCs).

Methods: Human placental chorionic tissues from term third-trimester pregnancies (n=3) were used. The cMSCs were cultured on rationally designed ECM protein mimetics coated with MAP on plastic surfaces with the addition of reduced fetal bovine serum (0.5%, 1% FBS). Adhesion capabilities were monitored by a real time cell analysis system (RTCA) utilizing an impedance method. Proliferation capabilities were monitored by RTCA and MTS assay.

Results: Of the ECM protein mimetics tested, GRGDSP(FN) coated surfaces exhibited the highest adhesion and proliferation capabilities on RTCA at FBS concentration of 0.5% and 1%. When 0.5% FBS was added to ECM protein mimetics during the MTS assay, GRGDSP(FN), REDV(FN), and collagen mimetics, GPKGAAGEPGKP(ColI) showed higher cMSCs proliferation compared with the control. When 1% FBS was added, GRGDSP(FN) and TAIPSCPEGTVPLYS(ColIV) showed significant cMSCs proliferation capacity.

Conclusions: Fibronectin mimetics, GRGDSP(FN) amino acid sequence showed the highest adhesion and proliferation capabilities. In addition, results from RTCA assessment of cell viability correlated well with the tetrazolium-based MTS assay.

Keywords: chorionic mesenchymal stem cell, extracellular matrix, protein mimetics, real time cell analysis, cell proliferation assay.

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How to cite this article:
Kim JH, Jekarl DW, Kim M, Oh EJ, Kim Y, Park IY, Shin JC. Effects of ECM Protein Mimetics on Adhesion and Proliferation of Chorion Derived Mesenchymal Stem Cells. Int J Med Sci 2014; 11(3):298-308. doi:10.7150/ijms.6672. Available from http://www.medsci.org/v11p0298.htm