International Journal of Medical Sciences

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13 December 2017

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Int J Med Sci 2014; 11(3):222-225. doi:10.7150/ijms.7382

Short Research Communication

Chymase Activities and Survival in Endotoxin-Induced Human Chymase Transgenic Mice

Kazi Rafiq1✉, Yu-Yan Fan1, Shamshad J. Sherajee1, Yoshimasa Takahashi2, Junji Matsuura2, Naoki Hase2, Hirohito Mori3, Daisuke Nakano1, Hideki Kobara3, Hirofumi Hitomi1, Tsutomu Masaki3, Hidenori Urata4, Akira Nishiyama1

1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
2. Teijin Institute for Bio-Medical Research, Teijin Pharma Ltd., Tokyo, Japan.
3. Department of Gastroenterology and Neurology, Kagawa University Medical School, Kagawa, Japan.
4. Department of Cardiovascular Diseases, Fukuoka University Chikushi Hospital, Fukuoka, Japan.

Abstract

We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not affect survival in either WT or Tg mice. WT mice were not affected by 0.1 mg/day of LPS, whereas 25% of Tg mice died. Survival of mice treated with 0.3 mg/day of LPS was 87.5% and 0% in WT and Tg, respectively. LPS-induced increases in chymase activity in the heart and skin were significantly greater in Tg than WT mice. These data suggest a possible contribution of human chymase activation to LPS-induced mortality.

Keywords: human chymase transgenic mice, chymase activity and lipopolysaccharide, endotoxemia

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How to cite this article:
Rafiq K, Fan YY, Sherajee SJ, Takahashi Y, Matsuura J, Hase N, Mori H, Nakano D, Kobara H, Hitomi H, Masaki T, Urata H, Nishiyama A. Chymase Activities and Survival in Endotoxin-Induced Human Chymase Transgenic Mice. Int J Med Sci 2014; 11(3):222-225. doi:10.7150/ijms.7382. Available from http://www.medsci.org/v11p0222.htm