International Journal of Medical Sciences

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19 October 2017

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Int J Med Sci 2013; 10(10):1344-1351. doi:10.7150/ijms.4802

Research Paper

Prolidase, Matrix Metalloproteinases 1 and 13 Activity, Oxidative-Antioxidative Status as a Marker of Preterm Premature Rupture of Membranes and Chorioamnionitis in Maternal Vaginal Washing Fluids

Hatice Ender Soydinc1✉, Muhammet Erdal Sak1, Osman Evliyaoglu2, Mehmet Sıddık Evsen1, Abdulkadir Turgut1, Ali Özler1, İsmail Yıldız3, Talip Gul1

1. Department of Gynecology and Obstetrics, Faculty of Medicine, Dicle University, Diyarbakir, Turkey
2. Department of Biochemistry, Faculty of Medicine, Dicle University, Diyarbakir, Turkey
3. Department of Bioistatistic, Faculty of Medicine, Dicle University, Diyarbakir, Turkey

Abstract

Objective: Etiology of premature preterm rupture of membranes (PPROM) is not yet completely known and chorioamnionitis is one of the most important complications of its. We aimed to evaluate whether prolidase, matrix metalloproteinases, oxidative-antioxidative status, and inflammation markers in vaginal washing fluid (VWF) were associated with etiology of PPROM and whether these markers could be used to predict chorioamnionitis in PPROM.

Study Design: This prospective case control study enrolled fifty pregnant women with PPROM and 50 healthy pregnant women. The VWF samples were taken at the time of admission in the PPROM group and patients were followed for chorioamnionitis. Prolidase, matrix metalloproteinases, oxidative-antioxidative status, and inflammation markers in VWF were assayed.

Results: VWF levels of prolidase, matrix metalloproteinases 1-13 (p< 0.001), oxidative stress parameters, total oxidative stress (TOS) (p < 0.001) and oxidative stress index (OSI) (p = 0.002), and hs-CRP (p = 0.045) were significantly higher in the PPROM group than in the controls. Antioxidative status parameters, levels of paroxanase (PON-1) (p < 0.001) and total antioxidant capacity (TAC) (p < 0.001), were significantly lower in the PPROM group than in the controls. Mean VWF levels of prolidase (p < 0.001), metalloproteinases (p<0.05), and oxidative-antioxidative status parameters (p<0.05) were significantly different in women with versus women without chorioamnionitis in the PPROM group. Prolidase, MMP-13, TOS, TAC, and PON-1 were found as important predictors for chorioamnionitis in the PPROM group by the multivariate logistic regression analysis. When the ROC curve analysis for prolidase, MMP-13, TOS, TAC, and PON-1 were performed, all of them were statistically significant for area under the curve (areas under the curve were 0.94, 0.90, 0.80, 0.25, and 0.19, respectively).

Conclusions: This study showed that collagen turnover mediators, especially prolidase, and increased oxidative stress are significantly associated with PPROM. Also, chorioamnionitis can be predicted with prolidase, MMP-13, TOS, TAC, and PON-1 in PPROM patients.

Keywords: premature preterm rupture of membrane, chorioamnionitis, prolidase, matrix metalloproteinases, total oxidative status, total antioxidative capacity, paraoxonase-1, vaginal washing fluid.

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How to cite this article:
Soydinc HE, Sak ME, Evliyaoglu O, Evsen MS, Turgut A, Özler A, Yıldız İ, Gul T. Prolidase, Matrix Metalloproteinases 1 and 13 Activity, Oxidative-Antioxidative Status as a Marker of Preterm Premature Rupture of Membranes and Chorioamnionitis in Maternal Vaginal Washing Fluids. Int J Med Sci 2013; 10(10):1344-1351. doi:10.7150/ijms.4802. Available from http://www.medsci.org/v10p1344.htm