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18 October 2017

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Int J Med Sci 2013; 10(1):60-67. doi:10.7150/ijms.5014

Research Paper

Mutation Screening of the BRCA1 Gene in Early Onset and Familial Breast/Ovarian Cancer in Moroccan Population

Abdelilah Laraqui1,7, Nancy Uhrhammer2, Idriss Lahlou-Amine1, Hicham EL Rhaffouli1, Jamila El Baghdadi3, Mohamed Dehayni4, Rahali Driss Moussaoui4, Mohamed Ichou5, Yassir Sbitti5, Abderrahman Al Bouzidi6, Said Amzazi7, Yves-Jean Bignon2✉

1. Laboratoire de Recherche et de Biosécurité P3, Hôpital Militaire d'Instruction Mohammed V, Rabat, Maroc;
2. Laboratoire Diagnostic Génétique et Moléculaire, Centre Jean Perrin, 58 rue Montalembert, Clermont-Ferrand 63011, France;
3. Unité Génétique, Hôpital Militaire d'Instruction Mohammed V, Rabat, Maroc;
4. Service de Gynécologie, Hôpital Militaire d'Instruction Mohammed V, Rabat, Maroc;
5. Service d'Oncologie, Hôpital Militaire d'Instruction Mohammed V, Rabat, Maroc;
6. Laboratoire d'Anatomopathologie, Hôpital Militaire d'Instruction Mohammed V, Equipe de recherche en pathologie tumorale, Faculté de médecine et de Pharmacie, Rabat, Maroc;
7. Laboratoires de Biochimie -Immunologie, Maroc, Université Mohammed V-Agdal, Faculté des Sciences de Rabat, Maroc.

Abstract

Worldwide variation in the distribution of BRCA mutations is well recognised, and for the Moroccan population no comprehensive studies about BRCA mutation spectra or frequencies have been published. We therefore performed mutation analysis of the BRCA1 gene in 121 Moroccan women diagnosed with breast cancer. All cases completed epidemiology and family history questionnaires and provided a DNA sample for BRCA testing. Mutation analysis was performed by direct DNA sequencing of all coding exons and flanking intron sequences of the BRCA1 gene. 31.6 % (6/19) of familial cases and 1 % (1/102) of early-onset sporadic (< 45 years) were found to be associated with BRCA1 mutations. The pathogenic mutations included two frame-shift mutations (c.798_799delTT, c.1016dupA), one missense mutation (c.5095C>T), and one nonsense mutation (c.4942A>T). The c.798_799delTT mutation was also observed in Algerian and Tunisian BC families, suggesting the first non-Jewish founder mutation to be described in Northern Africa. In addition, ten different unclassified variants were detected in BRCA1, none of which were predicted to affect splicing. Most unclassified variants were placed in Align-GVGD classes suggesting neutrality. c.5117G>C involves a highly conserved amino acid suggestive of interfering with function (Align-GVGD class C55), but has been observed in conjunction with a deleterious mutation in a Tunisian family. These findings reflect the genetic heterogeneity of the Moroccan population and are relevant to genetic counselling and clinical management. The role of BRCA2 in BC is also under study.

Keywords: Breast cancer, BRCA1 mutations, unclassified variants.

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How to cite this article:
Laraqui A, Uhrhammer N, Lahlou-Amine I, EL Rhaffouli H, El Baghdadi J, Dehayni M, Moussaoui RD, Ichou M, Sbitti Y, Al Bouzidi A, Amzazi S, Bignon YJ. Mutation Screening of the BRCA1 Gene in Early Onset and Familial Breast/Ovarian Cancer in Moroccan Population. Int J Med Sci 2013; 10(1):60-67. doi:10.7150/ijms.5014. Available from http://www.medsci.org/v10p0060.htm