Int J Med Sci 2012; 9(6):480-487. doi:10.7150/ijms.4583

Research Paper

Osteogenic Differentiation of Dental Follicle Stem Cells

Giorgio Mori1, Andrea Ballini2, Claudia Carbone3, Angela Oranger3, Giacomina Brunetti3, Adriana Di Benedetto3, Biagio Rapone2, Stefania Cantore2, Mariasevera Di Comite3, Silvia Colucci3, Maria Grano3, Felice R. Grassi2 ✉

1. Department of Biomedical Sciences, Medical School, University of Foggia, ITALY;
2. Department of Gynecology, Obstetrics and Neonatology, Faculty of Medicine and Surgery, University of Bari “Aldo Moro”, ITALY;
3. Department of Basic Medical Sciences, Section of Human Anatomy and Histology, University of Bari “Aldo Moro”, ITALY.


Background: Stem cells are defined as clonogenic cells capable of self-renewal and multi-lineage differentiation. A population of these cells has been identified in human Dental Follicle (DF).

Dental Follicle Stem Cells (DFSCs) were found in pediatric unerupted wisdom teeth and have been shown to differentiate, under particular conditions, into various cell types of the mesenchymal tissues.

Aim: The aim of this study was to investigate if cells isolated from DF show stem features, differentiate toward osteoblastic phenotype and express osteoblastic markers.

Methods: We studied the immunophenotype of DFSCs by flow cytometric analysis, the osteoblastic markers of differentiated DFSCs were assayed by histochemical methods and real-time PCR.

Results: We demonstrated that DFSCs expressed a heterogeneous assortment of makers associated with stemness. Moreover DFSCs differentiated into osteoblast-like cells, producing mineralized matrix nodules and expressed the typical osteoblastic markers, Alkaline Phosphatase (ALP) and Collagen I (Coll I).

Conclusion: This study suggests that DFSCs may provide a cell source for tissue engineering of bone.

Keywords: dental follicle, stem cells, osteogenic markers, bone tissue engineering, human postnatal dental tissue.

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How to cite this article:
Mori G, Ballini A, Carbone C, Oranger A, Brunetti G, Di Benedetto A, Rapone B, Cantore S, Di Comite M, Colucci S, Grano M, Grassi FR. Osteogenic Differentiation of Dental Follicle Stem Cells. Int J Med Sci 2012; 9(6):480-487. doi:10.7150/ijms.4583. Available from