Int J Med Sci 2012; 9(1):51-58. doi:10.7150/ijms.9.51
The GHS-R Blocker D-[Lys3] GHRP-6 Serves as CCR5 Chemokine Receptor Antagonist
Laboratory of Molecular Biology and Immunology, National Institute on Aging, Intramural Program, NIH, 251 Bayview Boulevard, Baltimore, MD. 21224, USA.
‡ Present Address: Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD. 21231.
† Present Address: Laboratory of Neuroendocrine-Immunology, Pennington Biomedical Research Center, LSU System, Baton Rouge LA. 70808.
* Present Address: BioSensors Branch, RDCB-DRB-S, Edgewood Chemical Biological Center, APGEA, MD. 21010.
# Present Address: BioLegend, 1180 Roselle St., San Diego, CA. 92121.
Patel K, Dixit VD, Lee JH, Kim JW, Schaffer EM, Nguyen D, Taub DD. The GHS-R Blocker D-[Lys3] GHRP-6 Serves as CCR5 Chemokine Receptor Antagonist. Int J Med Sci 2012; 9(1):51-58. doi:10.7150/ijms.9.51. Available from http://www.medsci.org/v09p0051.htm
[D-Lys3]-Growth Hormone Releasing Peptide-6 (DLS) is widely utilized in vivo and in vitro as a selective ghrelin receptor (GHS-R) antagonist. This antagonist is one of the most common antagonists utilized in vivo to block GHS-R function and activity. Here, we found that DLS also has the ability to modestly block chemokine function and ligand binding to the chemokine receptor CCR5. The DLS effects on RANTES binding and Erk signaling as well as calcium mobilization appears to be much stronger than its effects on MIP-1α and MIP-1β. CCR5 have been shown to act as major co-receptor for HIV-1 entry into the CD4 positive host cells. To this end, we also found that DLS blocks M-tropic HIV-1 propagation in activated human PBMCs. These data demonstrate that DLS may not be a highly selective GHS-R1a inhibitor and may also effects on other G-protein coupled receptor (GPCR) family members. Moreover, DLS may have some potential clinical applications in blocking HIV infectivity and CCR5-mediated migration and function in various inflammatory disease states.
Keywords: MIP-1α, MIP-1β, RANTES, CCR5, HIV-1, Ghrelin, GHRP-6, GHS-R1a, Inflammation, Cancer.
Chemokines are small peptides that are known to exert potent regulatory effects on migration and activation of various immune and non hematopoietic cells via ligation to their seven transmembrane G-protein coupled receptors [1, 2]. CCR5 serves as an endogenous ligand for CC chemokines, MIP-1α/CCL3, MIP-1β/CCL4 and RANTES/CCL5. CC chemokine receptor 5 (CCR5) regulates trafficking and effector functions of memory/effector T-lymphocytes, macrophages, and immature dendritic cells . Interestingly, chemokine receptors CXCR4 and CCR5 have attracted substantial interest because they form portals of cellular entry for the human immunodeficiency viruses (HIV-1 and HIV-2) and related simian or feline retroviruses. While all the HIV-1 strains require CD4 to enter and infect cells, some use the chemokine receptor CXCR4 (T-tropic/X4 strain or syncytium-inducing viruses), or CCR5 (M-tropic/R5 strain or non-syncytium-inducing viruses) and some can utilize either coreceptor (R5X4 strains) for these purposes. Several HIV-1 coreceptor antagonists have been recently discovered and are being utilized in human clinical trials. In addition to pathogenesis of HIV, CCR5 receptors have been implicated in motility . Given the involvement of CCR5 in HIV and inflammation these receptors have emerged as potential targets for therapeutic intervention .
Growth hormone secretagogue receptor (GHS-R) belongs to a seven transmembrane GPCR family and serves as an endogenous ligand for stomach derived hormone ghrelin [6, 7]. Growth hormone releasing peptide-6 (GHRP-6) is one of earliest synthetic peptidyl GHS-R agonist utilized to study the functions of GHS-R prior to the discovery of the endogenous ligand ghrelin. Modification of GHRP-6 (H-His-D-Trp-Ala-Trp-D-Phe-Lys-NH2) from alanine to D-lysine resulted in a GHS-R antagonist D-[Lys3]GHRP-6 (H-His-D-Trp-D-Lys-Trp-D-Phe-Lys-NH2). Currently, the D-[Lys3]GHRP-6 (DLS) is utilized in in vitro and in vivo studies as a selective GHS-R antagonist (Figure 1). However, no studies have yet addressed the specificity and efficacy of this compound in human T lymphocytes, T cell lines, PBMCs or other immune cell subsets. Given the potent effects of natural GHS-R ligand ghrelin on human T cell responses , we evaluated the specificity of DLS and its potential interactions with other immunologically relevant GPCRs of chemokine family and present evidence that DLS also modestly antagonizes CCR5 receptor signaling, function and HIV-1 coreceptor activity. The most important finding in these studies if not the discovery of DLS as a potential HIV antagonist (as other more potent and selective chemokine receptor antagonist are more efficient and selective) but that the DLS antagonist is not as highly selective to GHS-R1a as originally thought and may have some impact on other G-protein coupled receptors (GPCRs) including chemokine receptors.
Materials and Methods
Cell culture and Cell lines
CEM.NKR-CCR5, 3T3.T4.CCR5, CCR5 receptor antagonist TAK779  and HIV-1Ba-L  were obtained from the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH ( CEM.NKR-CCR5 from Dr. Alexandra Trkola, 3T3.T4.CCR5 from Dr. Dan R. Littman, HIV-1Ba-L from Dr. Suzanne Gartner, Dr. Mikulas Popovic and Dr. Robert Gallo). Pheresis packs were prepared from 4 healthy male donors between 18 and 45 years age for the isolation of PBMCs. PBMCs were obtained by Ficoll-Hypaque density centrifugation. PBMCs were activated with PHA.
Intracellular calcium mobilization
Measurement of intracellular calcium release in response to MIP-1α (60nM), MIP-1β (60nM) and RANTES (10nM) were performed as described previously and as described in the legends . CEM.NKR-CCR5 cells were incubated in PBS containing 5 mM Fura-2 acetoxymethyl ester (Molecular Probes) for 30 minutes at room temperature. The cells were subsequently washed and then resuspended at 1 x 106cells per ml in PBS. A total of 2 ml of the cell suspension was placed in a continuously stirring cuvette at room temperature in an LS50B spectrophotometer (Perkin-Elmer, Wellesley, Massachusetts, USA). Cells were treated with MIP-1α (60nM), MIP-1β (60nM)and RANTES (10nM) and along with DLS (Sigma-Aldrich) at various concentrations. Fluorescence was monitored at λex1 = 340 nm, λex2 = 380 nm, and λem = 510 nm. The data are presented as the relative ratio of fluorescence excited at 340 and 380 nm.
Fluorokine ligand binding
Fluorokine binding assay was performed as described previously and in the Figure legends . Briefly, biotinylated MIP-1α, MIP-1β and RANTES (Fluorokine; R&D Systems) staining was performed according to R&D Systems' protocols, with slight modifications. The control or treated CEM.NKR-CCR5 cells were resuspended in PBS at 4 x 106cells per ml. 25μl of cells were treated with 1μg, 4μg or 16 μg of DLS at 37°C for 30 min, then mixed with 20 µl of 2.5 µg/ml biotinylated SDF-1α and incubated at 4°C for 1 h. 20μl fluorescein-conjugated avidin (10 µg/ml) was added to the cells and incubated for an additional 30 min at 4°C. After incubation, cells were washed with 1x RDF-1 buffer (R&D Systems) and then fixed with 2% paraformaldehyde in PBS before being analyzed on a FACScan (BD Biosciences).
Western blot analysis
As described here and in the Figure legends, control and treated 3T3.T4.CCR5 cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and protein concentrations of cell lysates were determined by Bradford assay. Protein lysates (30μg) were diluted with sample buffer and separated on 4-20% Tris HCl SDS-polyacrylamide gels (Biorad, Hercules, CA) and electrophoretically transferred to nitrocellulose membranes (Schleicher & Schuell). The blots were then incubated with rabbit anti-phospho-P42 and pERK (Cell Signaling, Beverly, MA). Immune complexes were visualized by incubation with either an anti-rabbit or an anti-mouse HRP-conjugated secondary antibody (Amersham, Piscataway, NJ). The immunoreactive band was visualized by enhanced chemiluminescence (Perkin-Elmer).
HIV-1Ba-L was propagated in activated PBMC cells and p24 was measured in sup by ELISA (SAIC, Frederick, USA). PBMCs were activated by 5μg/ml phytoheamagglutinin (PHA) and 10units/ml IL2 for three days, on day three, cells were washed and resuspended as one million per ml in cRPMI medium. Activated PBMCs were treated with various concentrations of DLS and TAK779 at 37C for 30 minutes. Then HIV-1Ba-L was added to a final concentration of p24 of 10ng/ml. Incubate at 37C for three hours and wash cells to remove virus, resuspend cells as 5e+5 cells per ml, add antagonist and plate as triplicates in 24 well plate. Collect sup at day 3, 6 and 9 and measure p24 level in sup by ELISA. Graphs show % of p24 level over virus only controls at day 6 and day 9.
D-Lys3-GHRP-6 inhibits MIP-1α, MIP-1β and RANTES induced intracellular calcium release from CEM.NKR-CCR5 cells
Ligation of seven transmembrane GPCRs typically results in calcium mobilization from the intracellular stores by generation of inositol triphosphate. MIP-1α, MIP-1β and RANTES binding to CCR5 is known to elicit a potent release of calcium from intracellular sources. The direct effects of DLS on MIP-1α, MIP-1β and RANTES induced calcium mobilization were evaluated on CEM.NKR-CCR5 cells labeled with Fura-2AM. MIP-1α (60nM), MIP-1β (60nM)and RANTES (10nM) caused a significant increase in intracellular calcium and this calcium flux was markedly inhibited in a dose dependent fashion by DLS (Figure 2). DLS inhibition of calcium release was seen more clearly with RANTES than with MIP-1α and MIP-1β. Interestingly, higher concentrations of DLS were typically needed to observe a reproducible effect on chemokine-induced intracellular calcium mobilization. TAK779 was able to almost completely block the binding of all three chemokines at concentrations less than 2 µM (data not shown), demonstrating TAK779 to be a much more potent and selective antagonist than the GHS-R1a antagonist being examined here.
D-Lys3-GHRP-6 inhibits SDF-1-, MIP-1α-, MIP-1β- and RANTES-induced intracellular calcium release from CEM-CCR5 cells. CEM-CCR5 cells were loaded with Fura-2-AM dye, washed, treated with different concentration of DLS for 20 minutes and 1.5 ml cells used to read for calcium release after addition of chemokine in luminescence spectrometer. Each graph shows the 340nm/380nm excitation ratio at 509nm absorption. It shows CEM-CCR5 cells' calcium release after addition of MIP-1β (60nM), MIP-1α (60nM) and RANTES (10nM). The data reveals that, DLS partially inhibits MIP1β, MIP1α and RANTES induced calcium release (range of 10-40% inhibition).(Click on the image to enlarge.)
DLS inhibits MIP-1α, MIP-1β and RANTES binding
To determine if DLS blocks CCR5 receptor, we utilized a whole cell ligand binding assay with a biotinylated form of MIP-1α, MIP-1β and RANTES, followed by binding of an avidin-fluorescein isothiocyanate (FITC) conjugate, and subsequent examination by using flow cytometric analysis. Upon DLS treatment the percent maximal binding of MIP-1α and RANTES in the CEM.NKR-CCR5 cells was reduced reproducibly by up to 40% in the donors tested but binding of MIP-1β was not dramatically affected (Figure 3) suggesting that DLS may differentially blocks chemokine ligand binding to the CCR5 receptor. As expected, TAK779 was able to almost completely block the binding of all three chemokines at concentrations less than 2µM (data not shown), demonstrating TAK779 to be a much more potent and selective antagonist than the GHS-R1a antagonist being examined here.
DLS selectively blocks chemokine ligand binding to receptor-positive cells. 25μlof 4 million/ml cells were incubated with or without different amount of DLS (1/4/16μg) or TAK779 (for CCR5 inhibition, 1μg) for 30 minutes at 4°C. Add 20 ml of biotinylated-chemokine and incubate for 1hr at 4°C. After incubation, 20 μl of avidin-fluoresceine was added to the wells and then the plate was incubated for 30 minutes at 4°C. The cells were subsequently washed with 1xRDF b/f. Fix cells with 2% PFA and run on FACScan. Graph shows fluorescence intensity for each treatment. Results reveal that, DLS blocks binding of MIP-1α and RANTES with 20%-40% range. DLS does not affect MIP-1β binding.(Click on the image to enlarge.)
DLS inhibits chemokine-mediated signaling in CCR5-transfected 3T3 cell lines
3T3.T4.CCR5 cells were grown overnight and then pretreated with DLS (20μM) or the CCR5 specific antagonist, TAK779 (2μM) for 30 minutes at 37°C after which 10nM of MIP-1β or RANTES were added to cultures. The cells harvested in RIPA after washing with cold PBS at the indicated times. The results (Figure 4) reveal that DLS partially blocks RANTES-induced ERK activation in 3T3.T4.CCR5 cells. Surprisingly, MIP-1β-induced ERK activation was not influenced by DLS using this cell line. Tak779 at 2µM only demonstrated a 20-30% inhibition of ERK phosphorylation in these CCR5 expressing cells.
DLS decreases HIV-1 propagation in vitro in activated PBMCs
Given the ability of DLS to partially abrogate chemokine binding and signaling, we sought to determine if DLS could exhibit any inhibitory effects in HIV-1 infectivity in human PBMCs. Activated PBMCs treated with DLS or the chemokine receptor antagonist TAK779 for 30 minutes at 37°C. HIV-1Ba-L (CCR5 tropic) was added to the cultures at 37°C for 3 hours. The non-bound virus was washed off the treated cells and the cells were cultured for 3, 6 or 9 days to examine HIV-1Ba-L viral output by the infected cells. Supernatants were collected from each well at day 3, 6 and 9 and p24 levels were measured using ELISA assays. The results reveal that DLS inhibits R5 tropic HIV-1Ba-L propagation in PBMCs (Figure 5).
DLS inhibits chemokine- mediated signaling in CCR5-transfected 3T3 cell lines. Approximately 200,000 3T3.T4.CCR5 cells were grown overnight per well in 6-well plate. On the following day, the media was replaced with 2ml of serum free medium. After 2-3 hrs, DLS (20μM) or the CCR5 specific antagonist, TAK779 (2μM), were added to respective wells, incubated for 30 minutes at 37°C after which 10nM of RANTES (A) or MIP-1β (B) was added to indicated wells. The cells harvested in RIPA after washing with cold PBS at the indicated times. 30μg of protein run in 4-20% Bis-Tris gel, transferred and probed with mouse anti-pERK antibody (upper panel) stripped and again probed with rabbit anti-total ERK antibody (lower panel). The results reveal that DLS partially blocks RANTES induced ERK activation but surprisingly does not block MIP-1β-induced ERK activation in 3T3.T4.CCR5 cells.(Click on the image to enlarge.)
DLS decreases HIV-1 propagation in vitro in activated PBMCs. Activated PBMCs were washed and treated with DLS or TAK779 for 30 minutes at 37°C. Add 10ng (p24) of HIV-1Ba-L (CCR5 tropic) per one million cells in one ml medium , incubate at 37°C for 3hrs. Wash cells to remove unbound virus and resuspended to a concentration of 0.5 million cells per one ml medium, add antagonists and plate in triplicate wells in 24-well plate and incubate at 37°C. Collect supernatant from each well at day 6 and 9 and measure p24 level using ELISA. The graphs show % of p24 level over virus only controls at day 6 & 9. The results reveal that DLS inhibits R5 tropic HIV-1 propagation in PBMCs.(Click on the image to enlarge.)
Migration of immune cells to sites of inflammation is a multistep process mediated largely by interactions of various chemokines to their G protein linked seven transmembrane receptors [1, 14]. CCR5 and CXCR4 are the principal chemokine receptors critical for cellular migration and are used in association with CD4 by human immunodeficiency virus (HIV) to enter its target cells. These co-receptors are important determinants of viral tropism, pathogenesis and virulence and are widely believed to be important drug targets to prevent HIV infections . A number of CCR5 inhibitors like SCH417690, UK427857, GSK873140, PRO140 are currently being tested for their efficacy against R5 tropic HIV viruses . The synthetic peptidyl compound D-[Lys3]GHRP-6 (H-His-D-Trp-D-Lys-Trp-D-Phe-Lys-NH2) is currently believed to be a selective antagonist of ghrelin receptors (GHS-R). However, no studies exist on the potential interaction of this compound with other clinically relevant GPCRs of chemokine family. We have discovered that DLS can also modestly antagonize the binding and signaling of CCR5 chemokine receptor, more potently acting on RANTES activity through CCR5 (Figure 6). DLS has been utilized experimentally in rodent models without any adverse side effects and repeated administration have been found to reduce body weight in obese mice and improve their glycaemic control and insulin resistance . Furthermore, DLS reduced the size of abdominal fat pads without affecting the muscle mass in these mice. There is accumulating clinical evidence linking currently used HIV inhibitors to the pathogenesis of insulin resistance, dyslipidemia, lipodystrophy and atherosclerosis in AIDS patients . Thus DLS along with its potential HIV inhibitory properties may attenuate the metabolic effects associated with HAART therapy in AIDS patients. Additionally, DLS does not affect food intake in the fed state when circulating ghrelin levels are low, allowing for its potential use post-prandially.
Summary of DLS and TAK779 activities on HIV-1 propagation and CCR5 Function. While the current and most efficient treatment modalities for the treatment of HIV-1+ patients include reverse transcriptase and protease inhibitors, viruses are now developing resistance to one or more agents. Despite the efficacy of these agents, there is still dire need to discover new anti-HIV agents to inhibit viral infectivity. CXCR4 and CCR5 are the major co-receptors for HIV-1 entry into the CD4 positive cells. Thus, it would appear that chemokine receptors, more specifically here CCR5, are attractive targets to block HIV-1 binding, fusion and infectivity. Several potent chemokine receptor antagonists have been developed and are being utilized in clinical studies. TAK779 is a very selective CCR5 blocking agent and has been shown to block HIV-1 entry through CCR5-bearing cells and also blocks CCR5 function and signaling. We have found that the GHS-R1a antagonist, DLS, exhibits some modest blocking effects on CCR5 ligand binding and signaling, with the greatest effects being observed on RANTES binding and activity. Moreover, DLS can inhibit CCR5-trophic HIV-1 infectivity in CCR5+ immune cells, although it is significantly less potent than the CCR5 selective antagonist, TAK779, which is currently being utilized in clinical trials. While DLS may possess some modest antagonist activity for CCR5, the primary finding focused on in this manuscript is that DLS, initially thought to be a highly selective antagonist for the ghrelin receptor, GHS-R1a, appears to be promiscuous and may have some effects on other G-protein coupled receptors (GPCRs) such as chemokine receptors.(Click on the image to enlarge.)
Therapy for HIV-1+ subjects includes reverse transcriptase and protease inhibitors. These inhibitors have achieved sustained suppression of viral replication in HIV-1-infected individuals, although these viruses are now developing resistance to one or more agents. These inhibitors are costly and require long term use. Despite the efficacy of these agents, there is still dire need to discover new anti-HIV agents to inhibit viral infectivity. CXCR4 and CCR5 are the major co-receptors for HIV-1 entry into the CD4 positive cells. Several of the SNPs and specific deletions in CXCR4 and CCR5 genes have been shown to result in a resistance or slower progression of HIV-1 infection without any substantial immune (functional) defect in humans. Thus, it would appear that chemokine receptors, more specifically CXCR4 and CCR5, are attractive targets to block HIV-1 binding, fusion and infectivity (Figure 6). TAK779 is a very selective CCR5  blocking agent. Surprisingly, this antagonist has been discontinued after phase I trial, because of severe toxicities .
We would like to thank Mr. Gary Collins for his assistance in the completion of this work. This work was supported by the Intramural Research Program of the National Institute on Aging, National Institutes of Health.
All authors read and approved the final manuscript. KP, VDD, JHL, JWK, EMS and DN performed the cell culture and isolation, flow cytometric analysis and ligand binding assays. KP performed all of the HIV infection and calcium mobilization assays. KP, JHL and JWK performed all of the Western blot analyses. KP, VDD, DZ and DDT played a role in the planning and design of the studies. KP, VDD, EMS, DN and DDT played a role in the writing and editing of the manuscript.
DLS, [D-Lys3]-Growth Hormone Releasing Peptide-6; FITC, fluorescein isothiocyanate; GPCRs, G-protein coupled receptors; GHRP-6, Growth Hormone Releasing Peptide-6; GHS-R, Growth Hormone Secretagogue Receptor; PBMCs, peripheral blood mononuclear cells; HIV, human immunodeficiency virus.
Conflict of Interest
The authors have declared that no conflict of interest exists.
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Corresponding author: Dennis D. Taub, PhD, Clinical Immunology Section, Laboratory of Molecular Biology and Immunology, National Institute on Aging-Intramural Research Program, NIH, 251 Bayview Boulevard, Baltimore, MD, 21224. Phone: (410) 558-8428; Fax: (410) 558-8284; E mail: Taubdnia.nih.gov.