International Journal of Medical Sciences

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20 October 2017

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Int J Med Sci 2005; 2(3):114-117. doi:10.7150/ijms.2.114

Short Research Communication

Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System

Qi Chun Toh, Tuan Lin Tan, Wei Qiang Teo, Chin Yee Ho, Subhajeet Parida, Wei Ning Chen

Hepatitis Viruses and Liver Cancer Research Laboratory, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive 05N-10, Singapore 637551

Abstract

Hepatitis B surface antigen (HBsAg) is the major component of the envelope of hepatitis B virus (HBV). As a resident membrane protein in the endoplasmic reticulum, it plays a key role in the viral morphogenesis. Little is known about cellular proteins that interact with HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane proteins have been isolated in this study. These include a resident protein of endoplasmic reticulum (thioredoxin-related transmembrane protein 2), an adaptor protein involved in clathrin-mediated endocytosis and HIV-mediated downregulation of CD4, and a co-receptor of coxsakie B virus. The significance of our findings is suggested by the identification of cellular membrane proteins interacting with other virus proteins. Further functional analysis of these HBsAg- interacting cellular membrane proteins should shed new insights on their role in HBV morphogenesis.

Keywords: HBsAg, Morphogenesis, Cellular Membrane Proteins, Split-ubiquitin Screening System

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How to cite this article:
Toh QC, Tan TL, Teo WQ, Ho CY, Parida S, Chen WN. Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System. Int J Med Sci 2005; 2(3):114-117. doi:10.7150/ijms.2.114. Available from http://www.medsci.org/v02p0114.htm