Int J Med Sci 2005; 2(2):64-69. doi:10.7150/ijms.2.64
Cell Cycle Arrest by a Natural Product via G2/M Checkpoint
Technology Development, CK Life Sciences Int'l Inc., 2 Dai Fu Street, Tai Po Industrial Estate, Hong Kong, China
CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric analysis, respectively. Specific cellular protein expressions were measured using Western blot analysis. Flow cytometric analysis indicated that CKBM induced G2/M cell cycle arrest and apoptosis, whereas differential protein expressions of p21, p53 and 14-3-3σ (stratifin) using Western blot analysis were enhanced. The differential expressions of p21, p53 and 14-3-3σ in AGS cancer cells after CKBM treatment may play critical roles in the G2/M cell cycle arrest that blocks cell proliferation and induces apoptosis.
Keywords: 14-3-3σ (stratifin), G2/M arrest, cell proliferation, checkpoint protein
Luk SCW, Siu SWF, Lai CK, Wu YJ, Pang SF. Cell Cycle Arrest by a Natural Product via G2/M Checkpoint. Int J Med Sci 2005; 2(2):64-69. doi:10.7150/ijms.2.64. Available from http://www.medsci.org/v02p0064.htm