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Int J Med Sci 2024; 21(8):1491-1499. doi:10.7150/ijms.96007 This issue Cite

Research Paper

Renal protective effects of Alpinate Oxyphyllae Fructus and mesenchymal stem cells co-treatment against D- galactose induced renal deterioration

Tsung-Jung Ho^1,2,3*, Tamilselvi Shanmugam^4*, Po-Hsiang Liao⁴, Marthandam Asokan Shibu⁴, William Shao-Tsu Chen^5,6, Kuan-Ho Lin⁷, Shang-Yeh Lu⁸, Chia-Hua Kuo⁹, Wei-Wen Kuo^10,11,12, Chih-Yang Huang^{4,13,14,15,16✉}

1. Department of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
2. Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
3. School of Post-Baccalaureate Chinese Medicine, College of Medicine, Tzu Chi University, Hualien, Taiwan.
4. Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
5. Department of Psychiatry, Tzu Chi General Hospital, 707, Section 3, Chung-Yang Road, Hualien 97004, Taiwan.
6. School of Medicine Tzu Chi University, 701, Section 3, Chung-Yang Road, Hualien 97004, Taiwan.
7. Department of Emergency Medicine, China Medical University Hospital, Taichung, Taiwan.
8. Division of Cardiovascular Medicine, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan.
9. Laboratory of Exercise Biochemistry, University of Taipei, Taiwan.
10. Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan.
11. Ph.D. Program for Biotechnology Industry, China Medical University, Taichung, Taiwan.
12. School of pharmacy, China Medical University, Taichung, Taiwan.
13. Department of Biological Science and Technology, Asia University, Taichung, Taiwan.
14. Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien 970, Taiwan.
15. Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
16. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
* Share equal contribution.

✉ Corresponding author: Chih-Yang Huang Ph.D. Chair Professor, Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan. Tel.: +886-3-8561825 ext 15930. Fax: +886-4-22032295. E-mail address: cyhuangcmu.edu.tw. More

Abstract

Age-related structural and functional changes in the kidney can eventually lead to development of chronic kidney disease, which is one of the leading causes of mortality among elderly people. For effective management of age-related kidney complications, it is important to identify new therapeutic interventions with minimal side-effects. The present study was designed to evaluate the synergistic effect of a traditional Chinese herb, Alpinate Oxyphyllae Fructus (AOF), and adipose-derived mesenchymal stem cells (ADMSCs) in ameliorating D-galactose (D-gal)-induced renal aging phenotypes in WKY rats. The study findings showed that D-gal-induced alteration in the kidney morphology was partly recovered by the AOF and ADMSC co-treatment. Moreover, the AOF and ADMSC co-treatment reduced the expression of proinflammatory mediators (NFkB, IL-6, and Cox2) and increased the expression of redox regulators (Nrf2 and HO-1) in the kidney, which were otherwise augmented by the D-gal treatment. Regarding kidney cell death, the AOF and ADMSC co-treatment was found to abolish the proapoptotic effects of D-gal by downregulating Bax and Bad expressions and inhibiting caspase 3 activation. Taken together, the study findings indicate that the AOF and ADMSC co-treatment protect the kidney from D-gal-induced aging by reducing cellular inflammation and oxidative stress and inhibiting renal cell death. This study can open up a new path toward developing novel therapeutic interventions using both AOF and ADMSC to effectively manage age-related renal deterioration.

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