ISSN: 1449-1907International Journal of Medical Sciences
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Int J Med Sci 2024; 21(7):1274-1279. doi:10.7150/ijms.93482 This issue Cite
Research Paper
Efficacy of Citicoline Delivered via Brain Extracellular Space against Experimental Acute Ischemic Stroke in Rats
1. Department of Geriatrics, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China.
2. Department of Radiology, China-Japan Friendship Hospital, Beijing 100029, China.
3. Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.
4. Beijing Key Laboratory of Magnetic Resonance Imaging Equipment and Technique, Beijing 100191, China.
5. Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Institute of Aging, Key Laboratory of Alzheimer's Disease of Zhejiang Province, Zhejiang Provincial Clinical Research Center for Mental Disorders, The Affiliated Wenzhou Kangning Hospital, School of Mental Health, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
6. Department of Neurology, Peking University Third Hospital, Beijing 100191, China.
7. Institute of Medical Technology, Peking University Health Science Center, Beijing 100191, China.
8. Department of Radiology, Peking University Third Hospital, Beijing 100191, China.
9. NMPA key Laboratory for Evaluation of Medical Imaging Equipment and Technique, Beijing 100191, China.
Abstract
Objective: Citicoline can be used to reduce acute ischemic stroke injury via venous infusion, however, its protective effects in the brain extracellular space remain largely unknown. Herein, we investigated the brain protective effects of citicoline administered via the brain extracellular space and sought precise effective dosage range that can protect against ischemic injury after experimental ischemic stroke in rats.
Methods: Fifty-six Sprague-Dawley rats were randomly divided into control, intraperitoneal (IP), caudate-putamen (CPu)-25, CPu-40, CPu-50, CPu-60 and CPu-75 groups based on the infusion site and concentration of citicoline. Two hours after the administration of citicoline, the rats were subjected to a permanent middle cerebral artery occlusion to mimic acute ischemic stroke. Then, the brain infarct volume in rats after stroke was measured and their neurological deficiency was evaluated to explain the protective effects and effective dosage range of citicoline.
Results: Compared to the control and IP groups, brain infarct volume of rats in CPu-40, CPu-50, and CPu-60 groups is significant smaller. Furthermore, the brain infarct volume of rats in CPu-50 is the least.
Conclusions: Here, we showed that citicoline can decrease the brain infarct volume, thus protecting the brain from acute ischemic stroke injury. We also found that the appropriate effective citicoline dose delivered via the brain extracellular space is 50 mM. Our study provides novel insights into the precise treatment of acute ischemic stroke by citicoline via the brain extracellular space, further guiding the treatment of brain disease.
Keywords: citicoline, acute ischemic stroke, extracellular space
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