Translational Potential of Baicalein in Mitigating RSL3-Induced Ferroptosis in Fibroblasts: Implications for Therapeutic Interventions

Kuo, Pao-Jen; Rau, Cheng-Shyuan; Wu, Yi-Chan; Tsai, Chia-Wen; Wu, Chia-Jung; Lin, Chia-Wei; Hsieh, Ching-Hua

doi:10.7150/ijms.91940

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Int J Med Sci 2024; 21(7):1257-1264. doi:10.7150/ijms.91940 This issue Cite

Research Paper

Translational Potential of Baicalein in Mitigating RSL3-Induced Ferroptosis in Fibroblasts: Implications for Therapeutic Interventions

Pao-Jen Kuo^1†, Cheng-Shyuan Rau^2†, Yi-Chan Wu¹, Chia-Wen Tsai¹, Chia-Jung Wu¹, Chia-Wei Lin¹, Ching-Hua Hsieh^1✉

1. Department of Plastic Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan.
2. Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan.
^† These authors contributed equally to this work as the first authors.

Citation:

Kuo PJ, Rau CS, Wu YC, Tsai CW, Wu CJ, Lin CW, Hsieh CH. Translational Potential of Baicalein in Mitigating RSL3-Induced Ferroptosis in Fibroblasts: Implications for Therapeutic Interventions. Int J Med Sci 2024; 21(7):1257-1264. doi:10.7150/ijms.91940. https://www.medsci.org/v21p1257.htm

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Abstract

Background: Ferroptosis is an iron-driven cell-death mechanism that plays a central role in various diseases. Recent studies have suggested that baicalein inhibits ferroptosis, making it a promising therapeutic candidate.

Materials and Methods: Fibroblast cultures were treated with different agents to determine the effects of baicalein on ferroptosis. Ferroptosis-related gene expression, lipid peroxidation, and post-treatment cellular structural changes were measured using real-time quantitative polymerase chain reaction, C11-BODIPY dye, and transmission electron microscopy, respectively.

Results: Baicalein significantly inhibited rat sarcoma virus selective lethal 3-induced ferroptosis in fibroblasts. Moreover, in baicalein-treated groups, reduced ferroptosis-related gene expression, decreased lipid peroxidation, and maintained cell structure was observed when compared with those of the controls.

Discussion: The ability of baicalein to counteract RSL3-induced ferroptosis underscores its potential protective effects, especially in diseases characterized by oxidative stress and iron overload in fibroblasts.

Conclusion: Baicalein may serve as a potent therapeutic agent against conditions in which ferroptosis is harmful. The compound's efficacy in halting RSL3-triggered ferroptosis in fibroblasts paves the way for further in vivo experiments and clinical trials.

Keywords: Baicalein, Rat sarcoma virus selective lethal 3 (RSL3), Ferroptosis, Fibroblasts, Nuclear factor erythroid 2-related factor

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APA

Kuo, P.J., Rau, C.S., Wu, Y.C., Tsai, C.W., Wu, C.J., Lin, C.W., Hsieh, C.H. (2024). Translational Potential of Baicalein in Mitigating RSL3-Induced Ferroptosis in Fibroblasts: Implications for Therapeutic Interventions. International Journal of Medical Sciences, 21(7), 1257-1264. https://doi.org/10.7150/ijms.91940.

ACS

Kuo, P.J.; Rau, C.S.; Wu, Y.C.; Tsai, C.W.; Wu, C.J.; Lin, C.W.; Hsieh, C.H. Translational Potential of Baicalein in Mitigating RSL3-Induced Ferroptosis in Fibroblasts: Implications for Therapeutic Interventions. Int. J. Med. Sci. 2024, 21 (7), 1257-1264. DOI: 10.7150/ijms.91940.

NLM

CSE

Kuo PJ, Rau CS, Wu YC, Tsai CW, Wu CJ, Lin CW, Hsieh CH. 2024. Translational Potential of Baicalein in Mitigating RSL3-Induced Ferroptosis in Fibroblasts: Implications for Therapeutic Interventions. Int J Med Sci. 21(7):1257-1264.

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