Osteopontin May Improve Postinjury Muscle Repair Via Matrix Metalloproteinases And tgf-β Activation in Regular Exercise

Wang, Yuchong; Hong, Liang; Jiang, Jingjing; Zhang, Xujun; Chen, Jianing; Diao, Hongyan

doi:10.7150/ijms.82925

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Int J Med Sci 2023; 20(9):1202-1211. doi:10.7150/ijms.82925 This issue Cite

Research Paper

Osteopontin May Improve Postinjury Muscle Repair Via Matrix Metalloproteinases And tgf-β Activation in Regular Exercise

Yuchong Wang, Liang Hong, Jingjing Jiang, Xujun Zhang, Jianing Chen, Hongyan Diao^✉

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.

Citation:

Wang Y, Hong L, Jiang J, Zhang X, Chen J, Diao H. Osteopontin May Improve Postinjury Muscle Repair Via Matrix Metalloproteinases And tgf-β Activation in Regular Exercise. Int J Med Sci 2023; 20(9):1202-1211. doi:10.7150/ijms.82925. https://www.medsci.org/v20p1202.htm

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Abstract

Skeletal muscle injuries are commonly observed during sports and trauma. Regular exercise promotes muscle repair; however, the underlying mechanisms require further investigation. In addition to exercise, osteopontin (OPN) contributes to skeletal muscle regeneration and fibrosis following injury. However, whether and how OPN affects matrix proteins to promote post-injury muscle repair remains uncertain. We recruited regular exercise (RE) and sedentary control (SC) groups to determine plasma OPN levels. Additionally, we developed a murine model of muscle contusion injury and compared the extent of damage, inflammatory state, and regeneration-related proteins in OPN knockout (OPN KO) and wild-type (WT) mice. Our results show that regular exercise induced the increase of OPN, matrix metalloproteinases (MMPs), and transforming growth factor-β (TGF-β) expression in plasma. Injured muscle fibers were repaired more slowly in OPN-KO mice than in WT mice. The expression levels of genes and proteins related to muscle regeneration were lower in OPN-KO mice after injury. OPN also promotes fibroblast proliferation, differentiation, and migration. Additionally, OPN upregulates MMP expression by activating TGF-β, which promotes muscle repair. OPN can improve post-injury muscle repair by activating MMPs and TGF-β pathways. It is upregulated by regular exercise. Our study provides a potential target for the treatment of muscle injuries and explains why regular physical exercise is beneficial for muscle repair.

Keywords: Osteopontin, Muscle repair, Matrix metalloproteinases, TGF-β, Regular exercise

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APA

Wang, Y., Hong, L., Jiang, J., Zhang, X., Chen, J., Diao, H. (2023). Osteopontin May Improve Postinjury Muscle Repair Via Matrix Metalloproteinases And tgf-β Activation in Regular Exercise. International Journal of Medical Sciences, 20(9), 1202-1211. https://doi.org/10.7150/ijms.82925.

ACS

Wang, Y.; Hong, L.; Jiang, J.; Zhang, X.; Chen, J.; Diao, H. Osteopontin May Improve Postinjury Muscle Repair Via Matrix Metalloproteinases And tgf-β Activation in Regular Exercise. Int. J. Med. Sci. 2023, 20 (9), 1202-1211. DOI: 10.7150/ijms.82925.

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CSE

Wang Y, Hong L, Jiang J, Zhang X, Chen J, Diao H. 2023. Osteopontin May Improve Postinjury Muscle Repair Via Matrix Metalloproteinases And tgf-β Activation in Regular Exercise. Int J Med Sci. 20(9):1202-1211.

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