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Int J Med Sci 2023; 20(9):1174-1188. doi:10.7150/ijms.86533 This issue Cite

Research Paper

Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage

Zhenzhen Liu1,2#, Yao Tang1#, Xiaoyue Zhang1,2#, Jiangnan Pei1, Chengjie Wang1, Haiyan Liu1, Yi Yu1, Shouling Luo1, Weirong Gu1✉

1. Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
2. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai 200011, China.
# (Z Liu, Y Tang, and X Zhang contributed equally to this work)

✉ Corresponding author: W Gu; Email: guweirongedu.cn.

Citation:
Liu Z, Tang Y, Zhang X, Pei J, Wang C, Liu H, Yu Y, Luo S, Gu W. Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage. Int J Med Sci 2023; 20(9):1174-1188. doi:10.7150/ijms.86533. https://www.medsci.org/v20p1174.htm
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Abstract

Graphic abstract

Recurrent miscarriage (RM) is a pregnancy complication associated with dysregulation of the maternal-fetal interface. We aimed to identify dysfunctional interactions between trophoblast cells and decidual immune cells in RM. We downloaded single-cell RNA sequencing (scRNA-seq) datasets (GSE214607) from the Gene Expression Omnibus (GEO) datasets for further analysis using the R software. The data comprised of paired placental and decidual tissues, including those from patients diagnosed with RM and matched healthy controls. A total of 22976 cells were identified in 11 cell types, including trophoblasts, immune cells, and other cells. We divided trophoblast cells into three types and analyzed their interactions with decidual immune cells. Additionally, we re-clustered NK&T cells and macrophages, identified differentially expressed genes (DEGs), enriched their functions, and compared the cell interactions with trophoblast cells in each cell type. Our single-cell atlas of the maternal-fetal interface revealed alterations in the cellular organization of the decidua and placenta, cell type-specific transcriptome, and cell communication between immune and non-immune cells in RM, which are critical for illuminating the pathophysiology of RM.

Keywords: recurrent miscarriage, maternal-fetal interface, single-cell RNA sequencing, trophoblast cells, immune cells

Citation styles

APA
Liu, Z., Tang, Y., Zhang, X., Pei, J., Wang, C., Liu, H., Yu, Y., Luo, S., Gu, W. (2023). Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage. International Journal of Medical Sciences, 20(9), 1174-1188. https://doi.org/10.7150/ijms.86533.

ACS
Liu, Z.; Tang, Y.; Zhang, X.; Pei, J.; Wang, C.; Liu, H.; Yu, Y.; Luo, S.; Gu, W. Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage. Int. J. Med. Sci. 2023, 20 (9), 1174-1188. DOI: 10.7150/ijms.86533.

NLM
Liu Z, Tang Y, Zhang X, Pei J, Wang C, Liu H, Yu Y, Luo S, Gu W. Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage. Int J Med Sci 2023; 20(9):1174-1188. doi:10.7150/ijms.86533. https://www.medsci.org/v20p1174.htm

CSE
Liu Z, Tang Y, Zhang X, Pei J, Wang C, Liu H, Yu Y, Luo S, Gu W. 2023. Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage. Int J Med Sci. 20(9):1174-1188.

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