Int J Med Sci 2023; 20(8):1000-1008. doi:10.7150/ijms.83986 This issue Cite

Research Paper

A Study on the Effects of Muscarinic and Serotonergic Regulation by Bojanggunbi-tang on the Pacemaker Potential of the Interstitial Cells of Cajal in the Murine Small Intestine

Na Ri Choi1, Haejeong Jeong1, Woo-Gyun Choi1, Jae-Woo Park2,3, Seok-Jae Ko2,3✉, Byung Joo Kim1✉

1. Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 50612, Republic of Korea.
2. Department of Clinical Korean Medicine, Graduate School of Kyung Hee University, Seoul 02447, Republic of Korea.
3. Department of Gastroenterology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.

Citation:
Choi NR, Jeong H, Choi WG, Park JW, Ko SJ, Kim BJ. A Study on the Effects of Muscarinic and Serotonergic Regulation by Bojanggunbi-tang on the Pacemaker Potential of the Interstitial Cells of Cajal in the Murine Small Intestine. Int J Med Sci 2023; 20(8):1000-1008. doi:10.7150/ijms.83986. https://www.medsci.org/v20p1000.htm
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Abstract

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In traditional Korean medicine, the 16-herb concoction Bojanggunbi-tang (BGT) is used to treat various gastrointestinal (GI) diseases. In this study, we investigated the regulatory mechanism underlying the influence of BGT on the interstitial cells of Cajal (ICCs), pacemaker cells in the GI tract. Within 12 h of culturing ICCs in the small intestines of mice, the pacemaker potential of ICCs was recorded through an electrophysiological method. An increase in the BGT concentration induced depolarization and decreased firing frequency. This reaction was suppressed by cholinergic receptor muscarinic 3 (CHRM3) antagonists, as well as 5-hydroxytryptamine receptor (5HTR) 3 and 4 antagonists. Nonselective cation channel inhibitors, such as thapsigargin and flufenamic acid, along with protein kinase C (PKC) and mitogen-activated protein kinase (MAPK) inhibitors, also suppressed the BGT reaction. Guanylate cyclase and protein kinase G (PKG) antagonists inhibited BGT, but adenylate cyclase and protein kinase A antagonists had no effect. In conclusion, we demonstrated that BGT acts through CHRM3, 5HTR3, and 5HTR4 to regulate intracellular Ca2+ concentrations and the PKC, MAPK, guanylate cycle, and PKG signaling pathways.

Keywords: Traditional medicine, Bojanggunbi-tang, gastrointestinal disease, interstitial cells of Cajal, pacemaker potential


Citation styles

APA
Choi, N.R., Jeong, H., Choi, W.G., Park, J.W., Ko, S.J., Kim, B.J. (2023). A Study on the Effects of Muscarinic and Serotonergic Regulation by Bojanggunbi-tang on the Pacemaker Potential of the Interstitial Cells of Cajal in the Murine Small Intestine. International Journal of Medical Sciences, 20(8), 1000-1008. https://doi.org/10.7150/ijms.83986.

ACS
Choi, N.R.; Jeong, H.; Choi, W.G.; Park, J.W.; Ko, S.J.; Kim, B.J. A Study on the Effects of Muscarinic and Serotonergic Regulation by Bojanggunbi-tang on the Pacemaker Potential of the Interstitial Cells of Cajal in the Murine Small Intestine. Int. J. Med. Sci. 2023, 20 (8), 1000-1008. DOI: 10.7150/ijms.83986.

NLM
Choi NR, Jeong H, Choi WG, Park JW, Ko SJ, Kim BJ. A Study on the Effects of Muscarinic and Serotonergic Regulation by Bojanggunbi-tang on the Pacemaker Potential of the Interstitial Cells of Cajal in the Murine Small Intestine. Int J Med Sci 2023; 20(8):1000-1008. doi:10.7150/ijms.83986. https://www.medsci.org/v20p1000.htm

CSE
Choi NR, Jeong H, Choi WG, Park JW, Ko SJ, Kim BJ. 2023. A Study on the Effects of Muscarinic and Serotonergic Regulation by Bojanggunbi-tang on the Pacemaker Potential of the Interstitial Cells of Cajal in the Murine Small Intestine. Int J Med Sci. 20(8):1000-1008.

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