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Int J Med Sci 2023; 20(3):376-384. doi:10.7150/ijms.77603 This issue Cite

Research Paper

Denervation Atrophy of Skeletal Muscle is Not Influenced by Numb Levels in Mice

Abdurrahman Aslan1,2, Lauren Harlow1, Xin-Hua Liu1,2, Rita De Gasperi1,5, William A. Bauman1,2,3, Marco Brotto6, Christopher P Cardozo1,2,3,4✉

1. National Center for the Medical Consequences of Spinal Cord Injury, James J. Peters VA.
2. Department of Medicine, Icahn School of Medicine at Mount Sinai.
3. Department of Rehabilitation Medicine, Icahn School of Medicine at Mount Sinai.
4. Mount Sinai Institute for Systems Biomedicine, Icahn School of Medicine at Mount Sinai.
5. Department of Psychiatry and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai.
6. Bone-Muscle Research Center, College of Nursing & Health Innovation, The University of Texas at Arlington, Arlington, TX 76019.

✉ Corresponding author: Christopher Cardozo, MD, Center for the Medical Consequences of Spinal Cord Injury, James J. Peters VA Medical Center, 130 West Kingsbridge Road, Bronx, NY 10468. E-mail: christopher.cardozoedu; Phone 1-718-584-9000; FAX More

Citation:
Aslan A, Harlow L, Liu XH, De Gasperi R, Bauman WA, Brotto M, Cardozo CP. Denervation Atrophy of Skeletal Muscle is Not Influenced by Numb Levels in Mice. Int J Med Sci 2023; 20(3):376-384. doi:10.7150/ijms.77603. https://www.medsci.org/v20p0376.htm
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Abstract

Graphic abstract

Skeletal muscle undergoes rapid and extensive atrophy following nerve transection though the underlying mechanisms remain incompletely understood. We previously showed transiently elevated Notch 1 signaling in denervated skeletal muscle that was abrogated by administration of nandrolone (an anabolic steroid) combined with replacement doses of testosterone. Numb is an adaptor molecule present in myogenic precursors and skeletal muscle fibers that is vital for normal tissue repair after muscle injury and for skeletal muscle contractile function. It is unclear whether the increase in Notch signaling observed in denervated muscle contributes to denervation and whether expression of Numb in myofibers slows denervation atrophy. To address these questions, the degree of denervation atrophy, Notch signaling, and Numb expression was studied over time after denervation in C57B6J mice treated with nandrolone, nandrolone plus testosterone or vehicle. Nandrolone increased Numb expression and reduced Notch signaling. Neither nandrolone alone nor nandrolone plus testosterone changed the rate of denervation atrophy. We next compared rates of denervation atrophy between mice with conditional, tamoxifen-inducible knockout of Numb in myofibers and genetically identical mice treated with vehicle. Numb cKO had no effect on denervation atrophy in this model. Taken together, the data indicate that loss of Numb in myofibers does not alter the course of denervation atrophy and that upregulation of Numb and blunting of the denervation-atrophy induced activation of Notch do not change the course of denervation atrophy.

Keywords: skeletal muscle, numb, denervation atrophy, nandrolone, aging, sarcopenia

Citation styles

APA
Aslan, A., Harlow, L., Liu, X.H., De Gasperi, R., Bauman, W.A., Brotto, M., Cardozo, C.P. (2023). Denervation Atrophy of Skeletal Muscle is Not Influenced by Numb Levels in Mice. International Journal of Medical Sciences, 20(3), 376-384. https://doi.org/10.7150/ijms.77603.

ACS
Aslan, A.; Harlow, L.; Liu, X.H.; De Gasperi, R.; Bauman, W.A.; Brotto, M.; Cardozo, C.P. Denervation Atrophy of Skeletal Muscle is Not Influenced by Numb Levels in Mice. Int. J. Med. Sci. 2023, 20 (3), 376-384. DOI: 10.7150/ijms.77603.

NLM
Aslan A, Harlow L, Liu XH, De Gasperi R, Bauman WA, Brotto M, Cardozo CP. Denervation Atrophy of Skeletal Muscle is Not Influenced by Numb Levels in Mice. Int J Med Sci 2023; 20(3):376-384. doi:10.7150/ijms.77603. https://www.medsci.org/v20p0376.htm

CSE
Aslan A, Harlow L, Liu XH, De Gasperi R, Bauman WA, Brotto M, Cardozo CP. 2023. Denervation Atrophy of Skeletal Muscle is Not Influenced by Numb Levels in Mice. Int J Med Sci. 20(3):376-384.

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