Int J Med Sci 2022; 19(5):893-900. doi:10.7150/ijms.73489 This issue Cite

Research Paper

Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients

Ching-Yao Tsai1,2,3, Chueh-Tan Chen1,4, Hsin-Han Wu5, Chen-Chung Liao6, Kate Hua5, Chung-Hua Hsu4,7, Chian-Feng Chen5✉

1. Department of Ophthalmology, Taipei City Hospital Zhongxing Branch, Taipei, Taiwan
2. Institute of Public Health, National Yang Ming Chiao Tung University, Taipei, Taiwan
3. Department of Business Administration, Fu Jen Catholic University, New Taipei City, Taiwan
4. Institute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan
5. Cancer Progression Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan
6. Metabolomics-Proteomics Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan
7. Department of Chinese Medicine, Taipei City Hospital, Linsen, Chinese Medicine, and Kunming Branch, Taipei, Taiwan

Citation:
Tsai CY, Chen CT, Wu HH, Liao CC, Hua K, Hsu CH, Chen CF. Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients. Int J Med Sci 2022; 19(5):893-900. doi:10.7150/ijms.73489. https://www.medsci.org/v19p0893.htm
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Abstract

Graphic abstract

Purpose: The alteration of the exosomal proteins in the aqueous humor (AH) is linked to the development of eye diseases. The goal of this study was to examine the exosomal protein profile of patients with age-related macular degeneration (AMD) to better understand their role in the pathogenesis of AMD.

Methods: Exosomes were isolated from the AH of 28 AMD and 25 control eyes. The quality, concentration, and size distribution of exosomes were measured using a nanoparticle tracking analysis system (NTA). Total exosomal proteins from each sample were purified and digested with trypsin for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.

Results: Based on LC-MS/MS analysis, we got 105 exosomal peptides from AMD and control patients. Gene ontology (GO) analysis in the biology process revealed that exosomal proteins of AMD were enriched in the lipoprotein metabolic process. T-test analysis revealed six exosomal proteins in patients with AMD were significantly different from controls. Comparing the exosomal protein profile of AMD patients who were receiving anti-VEGF therapy, we observed the amount of two proteins decreased with the duration of the anti-VEGF treatment time.

Conclusions: In this study, we successfully isolated and purified AH exosomes. Our results provide pioneering findings for the exosomal protein profile in AMD development and under therapy. These unique proteins could be the new targets for drug discovery or biological markers for evaluating therapeutic efficacy.

Keywords: Aqueous Humor, Age-related macular degeneration, Exosome


Citation styles

APA
Tsai, C.Y., Chen, C.T., Wu, H.H., Liao, C.C., Hua, K., Hsu, C.H., Chen, C.F. (2022). Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients. International Journal of Medical Sciences, 19(5), 893-900. https://doi.org/10.7150/ijms.73489.

ACS
Tsai, C.Y.; Chen, C.T.; Wu, H.H.; Liao, C.C.; Hua, K.; Hsu, C.H.; Chen, C.F. Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients. Int. J. Med. Sci. 2022, 19 (5), 893-900. DOI: 10.7150/ijms.73489.

NLM
Tsai CY, Chen CT, Wu HH, Liao CC, Hua K, Hsu CH, Chen CF. Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients. Int J Med Sci 2022; 19(5):893-900. doi:10.7150/ijms.73489. https://www.medsci.org/v19p0893.htm

CSE
Tsai CY, Chen CT, Wu HH, Liao CC, Hua K, Hsu CH, Chen CF. 2022. Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients. Int J Med Sci. 19(5):893-900.

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