Int J Med Sci 2021; 18(16):3728-3737. doi:10.7150/ijms.64253 This issue

Research Paper

Long noncoding RNA CEBPA-DT promotes cisplatin chemo-resistance through CEBPA/BCL2 mediated apoptosis in oral squamous cellular cancer

Xue Qiao1,2, Jiayi Liu3, Li Zhu1,2, Rongbo Song1,2, Ming Zhong1,4✉, Yan Guo1,2✉

1. Department of Central Laboratory, School and Hospital of Stomatology, China Medical University, Liaoning Province Key Laboratory of Oral Disease.
2. Department of Oral Biology, School and Hospital of Stomatology, China Medical University, Liaoning Province Key Laboratory of Oral Disease.
3. Department of Oral Pathology, School and Hospital of Stomatology, China Medical University, Liaoning Province Key Laboratory of Oral Disease.
4. Department of Stomatology, Xiang'an Hospital of Xiamen University, Xiamen, China.

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Citation:
Qiao X, Liu J, Zhu L, Song R, Zhong M, Guo Y. Long noncoding RNA CEBPA-DT promotes cisplatin chemo-resistance through CEBPA/BCL2 mediated apoptosis in oral squamous cellular cancer. Int J Med Sci 2021; 18(16):3728-3737. doi:10.7150/ijms.64253. Available from https://www.medsci.org/v18p3728.htm

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Abstract

Graphic abstract

Intrinsic or developing resistance to chemotherapy drugs including cisplatin (CDDP) remains the major limitation of cancer therapeutic efficacy in cancers. Recently, increasing evidence suggested that long noncoding RNAs (lncRNAs) play a critical role in various biological processes of tumors, and have been implicated in resistance to various drugs. However, the role of lncRNAs in cisplatin resistance is poorly understood. Here, we found that the expression of lncRNA CEBPA-DT/CEBPA/BCL2 was upregulated in cisplatin resistance OSCC cells (Cal27-CisR and HSC4-CisR) compared with their parental cells (Cal27 and HSC4). CEBPA-DT overexpression could upregulated both cytoplasmic and nuclear CEBPA expression. Down-regulation of CEBPA-DT enhances cisplatin sensitivity, facilitates cell apoptosis in cisplatin-resistant OSCC cells. In addition, we identified that CEBPA-DT regulates cisplatin chemosensitivity through CEBPA/BCL2-mediated cell apoptosis. Knockdown of CEBPA and BCL2 could alleviate the increasement of cisplatin resistance induced by CEBPA-DT overexpression. Our findings indicate that downregulation of lncRNA CEBPA-DT may be a potential therapy to overcome cisplatin resistance in OSCC.

Keywords: oral squamous cell carcinoma, cisplatin, CEBPA Divergent Transcript, CEBPA, apoptosis