Int J Med Sci 2021; 18(9):2086-2092. doi:10.7150/ijms.54644 This issue Cite

Research Paper

Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration

Sheng-Huang Chang1*, Jer-Yuh Liu2,3*, Meen-Woon Hsiao4, Hsin‐Ling Yang5, Guan-Wei Wang4, Je-Chiuan Ye6,7✉

1. Tsaotun Psychiatric Center, Ministry of Health and Welfare, Nantou, Taiwan.
2. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
3. Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan.
4. School of Applied Chemistry, Chung-Shan Medical University, Taichung, Taiwan
5. Institute of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.
6. Department of Bachelor's Degree Program for Indigenous Peoples in Senior Health and Care Management, National Taitung University, Taitung, Taiwan.
7. Master Program in Biomedical Science, National Taitung University, Taitung, Taiwan.
* These authors have equal contribution.

Citation:
Chang SH, Liu JY, Hsiao MW, Yang H, Wang GW, Ye JC. Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration. Int J Med Sci 2021; 18(9):2086-2092. doi:10.7150/ijms.54644. https://www.medsci.org/v18p2086.htm
Other styles

File import instruction

Abstract

Graphic abstract

Ultraviolet C (UVC) has been applied to treatment of infections in wounds for at least the last two decades, however, cells being treated can be damaged if exposure is prolonged, which calls for protective measures, such as drug or herbal pre-treatment, to minimize damage. Ocimum gratissimum contains plant polyphenols such as isoflavones and caffeic acid, which have antioxidant effects. We hypothesize that Ocimum gratissimum aqueous extracts (OGE) can inhibit UVC-induced oxidative damage on skin cells. In this study, HaCaT skin cells are used to test the protective effects of OGE on cell proliferation and migration after exposure to UVC radiation. Pretreatment with OGE (50~150μg/mL) before 40 J/m2 UVC exposure was able to restore survival from 32.25% to between 46.77% and 68.00%, and 80 J/m2 UVC exposure from 11.49% to between 19.07% and 43.04%. Morphological observation of primarily apoptotic cell death confirms the above findings. The flow cytometry analysis revealed that UVC increased the number of cells at the sub-G1 phase in a dose dependent manner, and when pre-treated with OGE the changes were partially reversed. Moreover, the wound healing test for observing migration showed that UVC 40-80 J/m2 decreased cell migration to 47-28% activity and 100 μg/mL OGE was able to restore cell activity to81-69% at day 3. Based on the above results, we suggest that OGE has a protective effect on UVC-induced inhibition of cell proliferation and migration of skin cells and thus has potential application in wound care.

Keywords: Ultraviolet C, Skin cells, proliferation, migration, Ocimum gratissimum.


Citation styles

APA
Chang, S.H., Liu, J.Y., Hsiao, M.W., Yang, H., Wang, G.W., Ye, J.C. (2021). Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration. International Journal of Medical Sciences, 18(9), 2086-2092. https://doi.org/10.7150/ijms.54644.

ACS
Chang, S.H.; Liu, J.Y.; Hsiao, M.W.; Yang, H.; Wang, G.W.; Ye, J.C. Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration. Int. J. Med. Sci. 2021, 18 (9), 2086-2092. DOI: 10.7150/ijms.54644.

NLM
Chang SH, Liu JY, Hsiao MW, Yang H, Wang GW, Ye JC. Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration. Int J Med Sci 2021; 18(9):2086-2092. doi:10.7150/ijms.54644. https://www.medsci.org/v18p2086.htm

CSE
Chang SH, Liu JY, Hsiao MW, Yang H, Wang GW, Ye JC. 2021. Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration. Int J Med Sci. 18(9):2086-2092.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image