Int J Med Sci 2020; 17(17):2790-2798. doi:10.7150/ijms.49236

Research Paper

Lipidomic profiling of chorionic villi in the placentas of women with chronic venous disease

Miguel A Ortega1,2,3, Miguel A Saez1,3,4, Felipe Sainz5,6, Oscar Fraile-Martínez1, Sandra García-Gallego2,7, Leonel Pekarek1, Coral Bravo5,8, Santiago Coca1,2,3, Melchor Álvarez- Mon1,2,3,9, Julia Buján1,2,3✉#, Natalio García-Honduvilla1,2,3#, Ángel Asúnsolo2,5,10#

1. Department of Medicine and Medical Specialties, Faculty of Medicine and Health Sciences, University of Alcalá, Alcalá de Henares, Madrid, Spain.
2. Ramón y Cajal Institute of Healthcare Research (IRYCIS), Madrid, Spain.
3. University Center for the Defense of Madrid (CUD-ACD), 28047 Madrid, Spain.
4. Pathological Anatomy Service, Central University Hospital of Defence-UAH Madrid, Spain.
5. Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá, Alcalá de Henares, Madrid, Spain.
6. Angiology and Vascular Surgery Service, Central University Hospital of Defence-UAH Madrid, Spain.
7. Department of Organic and Inorganic Chemistry, and Research Institute in Chemistry “Andrés M. del Río” (IQAR), University of Alcalá, 28805 Madrid, Spain.
8. Service of Gynecology and Obstetrics, Section of Fetal Maternal Medicine, Central University Hospital of Defence-UAH Madrid, Spain.
9. Internal Medicine and Oncology Service Service, University Hospital Príncipe de Asturias, CIBEREHD, Alcalá de Henares, Madrid, Spain.
10. Department of Epidemiology & Biostatistics, Graduate School of Public Health and Health Policy, University of New York, New York, NY, United States.
#These authors shared senior authorship in this work.

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Citation:
Ortega MA, Saez MA, Sainz F, Fraile-Martínez O, García-Gallego S, Pekarek L, Bravo C, Coca S, Mon MÁ, Buján J, García-Honduvilla N, Asúnsolo Á. Lipidomic profiling of chorionic villi in the placentas of women with chronic venous disease. Int J Med Sci 2020; 17(17):2790-2798. doi:10.7150/ijms.49236. Available from http://www.medsci.org/v17p2790.htm

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Abstract

Background: Chronic venous disease (CVD) is a prevalent lower limb venous pathology that especially affects women, who also show an increased risk of this disease during pregnancy. Studies have shown significant structural changes in the placentas of women with CVD and several markers of tissue damage have been also described.

Patients and Methods: To try to understand the different placental pathologies, research efforts have focused on examining metabolomic profiles as indicators of the repercussions of these vascular disorders. This study examines changes produced in the metabolomic profiles of chorionic villi in the placentas of women with CVD. In a study population of 12 pregnant women, 6 with and 6 without CVD, we compared through mass spectroscopy coupled to ultra-high performance liquid chromatography (UHPLC-MS), 240 metabolites in chorionic villus samples.

Results: This study is the first to detect in the placental villi of pregnant women with CVD, modifications in lysophosphatidylcholines and amino acids along with diminished levels of other lipids such as triglycerides, sphingomyelins, and non-esterified omega 9 fatty acids, suggesting a role of these abnormalities in the pathogenesis of CVD.

Conclusions: Our findings are a starting point for future studies designed to examine the impacts of CVD on maternal and fetal well-being.

Keywords: Chronic venous disease, pregnancy, chorionic villi, triglycerides, sphingomyelins or non-esterified omega 9 fatty acids