Int J Med Sci 2020; 17(13):1992-2001. doi:10.7150/ijms.44723

Research Paper

MiR-145 functions as a tumor suppressor in Papillary Thyroid Cancer by inhibiting RAB5C

Wei Zhang1, Wenyue Ji2, Tianshu Li2, Ting Liu2, Xudong Zhao2✉

1. Department of Endocrinology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
2. Department of Otolaryngology head and neck surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, China.

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Citation:
Zhang W, Ji W, Li T, Liu T, Zhao X. MiR-145 functions as a tumor suppressor in Papillary Thyroid Cancer by inhibiting RAB5C. Int J Med Sci 2020; 17(13):1992-2001. doi:10.7150/ijms.44723. Available from http://www.medsci.org/v17p1992.htm

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Abstract

Papillary thyroid carcinoma (PTC) accounts for the largest proportion of thyroid cancers; and its morbidity rate has dramatically increased in recent decades. However, the pathogenesis mechanisms of PTC are still not clear. This study aimed to reveal that miR-145 acts as an antitumor miRNA in the progression of PTC. In the present study, the expression of miR-145 was analyzed in 57 paired PTC patient samples. The relationship between clinicopathological features and miR-145 expression were also defined. The tumor suppressive function of miR-145 on PTC cell metastasis, proliferation and apoptosis were revealed in vitro. Also, we used dual luciferase reporter assay to define the relationship of miR-145 and RAB5C. RAB5C was reported to participate in cell invasion and cell motility. We found that miR-145 was downregulated in PTCs, which was negatively correlated with PTC progression and metastasis. MiR-145 inhibited PTC migration, proliferation and promoted apoptosis by directly suppresing RAB5C. In conclusion, miR-145 functions as a tumor suppressor in PTC by inhibiting RAB5C. MiR-145 and RAB5C are potential therapeutic targets in therapy of aggressive PTC cases.

Keywords: miR-145, RAB5C, papillary thyroid carcinoma, PTC