Int J Med Sci 2020; 17(12):1819-1832. doi:10.7150/ijms.42612

Research Paper

Resistance training promotes reduction in Visceral Adiposity without improvements in Cardiomyocyte Contractility and Calcium handling in Obese Rats

Alexandre Barroso Melo1#, Andressa Prata Leite Damiani2#, Priscila Murucci Coelho2, Arícia Leone Evangelista Monteiro de Assis3, Breno Valentim Nogueira3, Lucas Guimarães Ferreira1, Richard Diego Leite1, Rogério Faustino Ribeiro Júnior4, Ana Paula Lima-Leopoldo1, André Soares Leopoldo1✉

1. Centre for Physical Education and Sports, Department of Sports, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.
2. Centre for Health Sciences, Department of Nutrition, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.
3. Center of Health Sciences, Department of Morphology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.
4. Center of Health Sciences, Department of Physiological Sciences, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.
#These authors contributed equally to this work.

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Citation:
Melo AB, Damiani APL, Coelho PM, de Assis ALEM, Nogueira BV, Guimarães Ferreira L, Leite RD, Ribeiro Júnior RF, Lima-Leopoldo AP, Leopoldo AS. Resistance training promotes reduction in Visceral Adiposity without improvements in Cardiomyocyte Contractility and Calcium handling in Obese Rats. Int J Med Sci 2020; 17(12):1819-1832. doi:10.7150/ijms.42612. Available from http://www.medsci.org/v17p1819.htm

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Abstract

Resistance training (RT) improves the cardiomyocyte calcium (Ca2+) cycling during excitation-contraction coupling. However, the role of RT in cardiomyocyte contractile function associated with Ca2+ handling in obesity is unclear. Wistar rats were distributed into four groups: control, sedentary obese, control plus RT, and obesity plus RT. The 10-wk RT protocol was used (4-5 vertical ladder climbs, 60-second interval, 3× a week, 50-100% of maximum load). Metabolic, hormonal, cardiovascular and biochemical parameters were determined. Reduced leptin levels, epididymal, retroperitoneal and visceral fat pads, lower body fat, and adiposity index were observed in RT. Obesity promoted elevation of collagen, but RT did not promote modifications of LV collagen in ObRT. RT induced elevation in maximum rates of contraction and relaxation, and reduction of time to 50% relaxation. ObRT group did not present improvement in the cardiomyocyte contractile function in comparison to Ob group. Reduced cardiac PLB serine16 phosphorylation (pPLB Ser16) and pPLB Ser16/PLB ratio with no alterations in sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) and phospholamban (PLB) expression were observed in Ob groups. Resistance training improved body composition reduced fat pads and plasma leptin levels but did not promote positive alterations in cardiomyocyte contractile function, Ca2+ handling and phospholamban phosphorylation.

Keywords: high-fat diet, obesity, resistance training, contractile function, calcium handling