Int J Med Sci 2020; 17(12):1665-1672. doi:10.7150/ijms.47273

Research Paper

Insensitive to PTH of CD8+ T cells regulate bone marrow mesenchymal stromal cell in aplastic anemia patients

Sidan Li1✉, Maoquan Qin1, Runhui Wu1, Hengxing Meng2, Yixuan He1, Bin Wang1, Xuan Zhou1, Guanghua Zhu1

1. Beijing Key Laboratory of Pediatric Hematology Oncology; National Key Discipline of Pediatrics, Ministry of Education; Key Laboratory of Major Diseases in Children, Ministry of Education; Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.
2. Zhong Wei Xin Biotechnology Co., Ltd, Tianjin, China.

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Citation:
Li S, Qin M, Wu R, Meng H, He Y, Wang B, Zhou X, Zhu G. Insensitive to PTH of CD8+ T cells regulate bone marrow mesenchymal stromal cell in aplastic anemia patients. Int J Med Sci 2020; 17(12):1665-1672. doi:10.7150/ijms.47273. Available from http://www.medsci.org/v17p1665.htm

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Abstract

Aplastic anemia (AA) is a rare disorder characterized by the suppression of bone marrow function resulting in progressive pancytopenia. The pathogenesis of AA is complex and involves an abnormal hematopoietic microenvironment, hematopoietic stem cell/progenitor cell deficiencies, and immunity disorders. However, the underlying mechanism of the disease is still not fully uncovered. In this research, we collected both donor and patient samples and found suppressed proliferation, abnormal differentiation as well as increased apoptosis of patient mesenchymal stem cells (MSCs). Considering the close relationship of parathyroid hormone (PTH) and MSCs differentiation, further studies showed that although patients maintained normal serum PTH level, their CD8+ T cells possessed lower PTH receptors. The insensitive to PTH of patients' CD8+ T cells finally lead to reduced expression of key Wnt factors. In all, bone marrow CD8+ T cells may play an important role in inducing MSCs adipogenesis and osteogenesis imbalancement.

Keywords: Aplastic anemia, mesenchymal stem cells, parathyroid hormone, CD8+ T cells