Int J Med Sci 2020; 17(1):125-136. doi:10.7150/ijms.35693

Research Paper

Transcriptome Profile of Human Fibroblasts in an Ex Vivo Culture

Bogusław Machaliński1✉, Dorota Rogińska1, Kamila Szumilas1, Alicja Zawiślak1, Aleksandra Wilk2, Iwona Stecewicz1, Andrzej Brodkiewicz1, Barbara Wiszniewska2

1. Department of General Pathology, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111 Szczecin, Poland
2. Department of Histology and Embryology, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111 Szczecin, Poland

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Citation:
Machaliński B, Rogińska D, Szumilas K, Zawiślak A, Wilk A, Stecewicz I, Brodkiewicz A, Wiszniewska B. Transcriptome Profile of Human Fibroblasts in an Ex Vivo Culture. Int J Med Sci 2020; 17(1):125-136. doi:10.7150/ijms.35693. Available from http://www.medsci.org/v17p0125.htm

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Abstract

Implantation of autologous fibroblasts is a method used to correct age-related changes in facial skin. The aim of this study was to establish the optimal population of cultured human fibroblasts according to the organization of the extracellular matrix in the dermis. Transcriptome profile analysis of cells derived from three consecutive passages indicated that fibroblasts after the second passage were the population with the greatest number of upregulated genes encoding the critical biological processes responsible for skin regeneration, such as extracellular matrix organization, collagen fibril organization, and cell adhesion. Furthermore, genes encoding proteinases responsible for the degradation of dermal extracellular matrix proteins were noticeably downregulated at this stage of culture. Autologous fibroblasts seem to be an optimal and safe biological filler for the renewal of all skin structures.

Keywords: autologous fibroblasts culture, transcriptome profile, gene expression