Int J Med Sci 2020; 17(1):63-70. doi:10.7150/ijms.36476 This issue
1. Department of Biochemistry, College of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
2. Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea.
3. Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 47392, Republic of Korea.
4. Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 47340, Republic of Korea.
The skin is one of the large organs in the human body and the most exposed to outdoor contaminants such as particulate matter < 2.5 µm (PM2.5). Recently, we reported that PM2.5 induced cellular macromolecule disruption of lipids, proteins, and DNA, via reactive oxygen species, eventually causing cellular apoptosis of human keratinocytes. In this study, the ethanol extract of Cornus officinalis fruit (EECF) showed anti-oxidant effect against PM2.5-induced cellular oxidative stress. EECF protected cells against PM2.5-induced DNA damage, lipid peroxidation, and protein carbonylation. PM2.5 up-regulated intracellular and mitochondrial Ca2+ levels excessively, which led to mitochondrial depolarization and cellular apoptosis. However, EECF suppressed the PM2.5-induced excessive Ca2+ accumulation and inhibited apoptosis. The data confirmed that EECF greatly protected human HaCaT keratinocytes from PM2.5-induced oxidative stress.
Keywords: ethanol extract of Cornus officinalis fruits (EECF), particulate matter 2.5 (PM2.5), human HaCaT keratinocytes, oxidative stress