Int J Med Sci 2020; 17(1):53-62. doi:10.7150/ijms.35977

Research Paper

Protective Effects of Resveratrol Supplementation on Contusion Induced Muscle Injury

Yi-Ju Hsu1, Chun-Shen Ho1,2, Mon-Chien Lee1, Chin-Shan Ho1, Chi-Chang Huang1, Nai-Wen Kan3✉

1. Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan;
2. Division of Physical Medicine and Rehabilitation, Lo-Hsu Foundation, Inc., Lotung Poh-Ai Hospital, Yilan 26546, Taiwan.
3. Center for General Education, Taipei Medical University, Taipei 11031, Taiwan.

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Citation:
Hsu YJ, Ho CS, Lee MC, Ho CS, Huang CC, Kan NW. Protective Effects of Resveratrol Supplementation on Contusion Induced Muscle Injury. Int J Med Sci 2020; 17(1):53-62. doi:10.7150/ijms.35977. Available from http://www.medsci.org/v17p0053.htm

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Abstract

Muscle injuries frequently occur in contact sports events. The current treatment options for soft tissue injuries remain suboptimal and often result in delayed or incomplete recovery of damaged muscles. Resveratrol (RES) is a phenolic phytochemical, well-known for its antioxidant and anti-inflammatory properties. The purpose of this study is to evaluate the potential beneficial effects of RES supplementation on inflammation and regeneration in skeletal muscle after a contusion injury, in comparison to a conventional treatment of nonsteroidal anti-inflammatory drugs (NSAID). After one week of acclimation, forty eight -week-old male ICR mice were randomly divided into the five groups (n=8 per group): 1) normal control (NC), 2) mass-drop injury without any treatment (mass-drop injury, MDI), 3) post-injury NSAID treatment (MDI+ 10mg/kg NSAID), 4) post-injury RES supplementation (MDI+ 25mg/kg/day RES) and 5) post-injury treatment with RES and NSAID (MDI + resveratrol+ NSAID). After muscle contusion injury of the left gastrocnemius muscle, RES or NSAID were orally administered post-injury once a day for 7 days. Results showed that the MDI group had significantly higher serum uric acid (UA), CREA (creatinine), LDH (lactic dehydrogenase) and creatine kinase (CK) than the normal control group. Treatment with resveratrol reduced muscle damage as evidenced by the significantly decreased serum levels of UA, CREA, LDH and CK after contusion-induced muscle injuries in mice. In addition, RES and RES + NSAID groups promoted muscle satellite cell regeneration with increase in desmin protein after injury. Our results suggest that resveratrol combined with NSAID potentially improve muscle recovery and may be a potential candidate for further development as an effective clinical treatment for muscle repair.

Keywords: mass-drop injury, regeneration, NSAID, LDH, CK