Int J Med Sci 2020; 17(1):45-52. doi:10.7150/ijms.35173

Research Paper

Molecular identification of isolates of the Trichophyton mentagrophytes complex

María Guadalupe Frías-De-León1,6, Erick Martínez-Herrera1,5,6, Carlos Enrique Atoche-Diéguez2, José Luís González- Cespón3, Brianda Uribe4, Roberto Arenas3,4,5,6, Carmen Rodríguez-Cerdeira3,5,6,7✉

1. Research Unit, High Speciality Regional Hospital of Ixtapaluca. Ixtapaluca, Edo. Mexico.
2. Dermatological Center of the Southeast “Dr. Fernando Latapí”, Mérida, Yucatán, México.
3. Efficiency, quality and costs in Health Services Research Group (EFISALUD), Galicia Sur Health Research Institute (IIS Galicia Sur). SERGAS-UVIGO.
4. Mycoloy Service, Hospital Manuel Gea González, Mexico City, Mexico.
5. Dermatology Department, Hospital do Meixoeiro and University of Vigo, Vigo, Spain.
6. European Women's Dermatologic and Venereologic Society (EWDVS), Tui, Spain.
7. Psychodermatology task force of the Ibero-Latin American College of Dermatology (CILAD).

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Citation:
Frías-De-León MG, Martínez-Herrera E, Atoche-Diéguez CE, Cespón JLG, Uribe B, Arenas R, Rodríguez-Cerdeira C. Molecular identification of isolates of the Trichophyton mentagrophytes complex. Int J Med Sci 2020; 17(1):45-52. doi:10.7150/ijms.35173. Available from http://www.medsci.org/v17p0045.htm

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Abstract

Background: The Trichophyton mentagrophytes complex is the second most common causal agent of dermatophytosis. It comprises five species—T. mentagrophytes, T. interdigitale, T. erinacei, T quinckeanum, and T. benhamie, as well as nine different genotypes of T. mentagrophytes / T. interdigitale—which are morphologically similar; however, their susceptibility to antifungal agents may differ. For targeted therapy and better prognosis, it is important to identify these species at a molecular level. However, since many hospitals lack molecular methods, the actual aetiology of dermatophytosis caused by this complex remains unknown.

Objective: To characterize 55 anthropophilic isolates of the T. mentagrophytes complex recovered from a dermatological centre in Yucatán, Mexico.

Material and methods: Fifty-five isolates of the T. mentagrophytes complex were obtained from patients with tinea capitis, tinea pedis, tinea corporis, tinea barbae, and tinea unguium. They were characterized by their colonial and microscopic morphology on Sabouraud dextrose agar (SDA) and through the sequencing of a fragment from the region ITS1-5.8S-ITS2.

Results: All colonies grown on SDA were white. Forty-six isolates formed colonies with a powdery texture, while nine isolates formed colonies with a velvety texture. The micromorphological features were typical of the T. mentagrophytes complex. The molecular analysis revealed that 55 isolates were microorganisms that belonged to the T. mentagrophytes complex, that 46 formed powdery colonies representing T. mentagrophytes, and that the other nine isolates that formed velvety colonies represented T. interdigitale. The latter nine isolates were obtained from patients with tinea pedis, tinea corporis, and tinea unguium.

Conclusions: The colony morphology on SDA led to the identification of 46 isolates as T. mentagrophytes and nine isolates as T. interdigitale. At a molecular level, the species identified by their morphology were identified only as T. mentagrophytes complex.

Keywords: Trichophyton mentagrophytes complex, Trichophyton mentagrophytes, Trichophyton interdigitale, Sabouraud dextrose agar, Internal transcribed spacer