Int J Med Sci 2019; 16(12):1621-1630. doi:10.7150/ijms.38502

Research Paper

Lipid emulsion inhibits the vasodilation induced by a toxic dose of amlodipine in isolated rat aortae

Seong-Ho Ok1,6#, Soo Hee Lee2#, Ji-Yoon Kim3, Hyun-Jin Kim4,5, Sung Il Bae3, Yeran Hwang3, Seongyeong Tak3, Ju-Tae Sohn2,6✉

1. Department of Anesthesiology and Pain Medicine, Gyeongsang National University Changwon Hospital, Changwon, 51427, Republic of Korea;
2. Department of Anesthesiology and Pain Medicine, Gyeongsang National University College of Medicine, Gyeongsang National University Hospital, 15 Jinju-daero 816 beon-gil, Jinju-si, Gyeongsangnam-do, 52727, Republic of Korea;
3. Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, 15 Jinju-daero 816 beon-gil, Jinju-si, Gyeongsangnam-do, 52727, Republic of Korea;
4. Division of Applied Life Sciences (BK21 plus), Gyeongsang National University, Gyeongsang, Republic of Korea;
5. Department of Food Science & Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Gyeongsang, Republic of Korea;
6. Institute of Health Sciences, Gyeongsang National University, Jinju-si, 52727, Republic of Korea.
#These authors contributed equally to the work.

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Citation:
Ok SH, Lee SH, Kim JY, Kim HJ, Bae SI, Hwang Y, Tak S, Sohn JT. Lipid emulsion inhibits the vasodilation induced by a toxic dose of amlodipine in isolated rat aortae. Int J Med Sci 2019; 16(12):1621-1630. doi:10.7150/ijms.38502. Available from http://www.medsci.org/v16p1621.htm

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Abstract

The goal of this study was to examine the effect of lipid emulsion on the vasodilation induced in isolated endothelium-denuded rat aortae by a toxic dose of amlodipine. We examined the effects of lipid emulsion and verapamil on amlodipine-induced vasodilation. We also examined the effects of a mixture of lipid emulsion and amlodipine, as well as the centrifuged aqueous extract (CAE) obtained by ultracentrifuging such a mixture and then removing the upper lipid layer, on amlodipine-induced vasodilation. The effect of lipid emulsion on the amlodipine concentration was examined. Lipid emulsion attenuated amlodipine-induced vasodilation in isolated aortae. Both CAE and lipid emulsion containing amlodipine inhibited amlodipine-induced vasodilation. However, there was no significant difference in amlodipine-induced vasodilation between aortae treated with CAE and those treated with lipid emulsion containing amlodipine. Verapamil inhibited amlodipine-induced vasodilation. Lipid emulsion decreased the concentration of amlodipine. Lipid emulsion attenuated the vasodilation induced by a toxic amlodipine dose in NaF-precontracted aortae. The data show that lipid emulsion inhibited the vasodilation induced by a toxic amlodipine dose in isolated rat aortae by reducing the concentration of amlodipine. Amlodipine-induced vasodilation seems to be mediated mainly by blockade of L-type calcium channels and partially by inhibition of the Rho-kinase pathway.

Keywords: amlodipine, lipid emulsion, centrifuged aqueous extract, vasodilation, verapamil