Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats

Tamaki, Hiroyuki; Yotani, Kengo; Ogita, Futoshi; Hayao, Keishi; Kirimto, Hikari; Onishi, Hideaki; Kasuga, Norikatsu; Yamamoto, Noriaki

doi:10.7150/ijms.32590

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Int J Med Sci 2019; 16(6):822-830. doi:10.7150/ijms.32590 This issue Cite

Research Paper

Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats

Hiroyuki Tamaki^1,2✉, Kengo Yotani², Futoshi Ogita², Keishi Hayao¹, Hikari Kirimto³, Hideaki Onishi¹, Norikatsu Kasuga⁴, Noriaki Yamamoto^1,5

1. Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, Japan
2. Department of Sports and Life Science, National Institute of Fitness and Sports in Kanoya, Japan
3. Department of Sensorimotor Neuroscience, Hiroshima University, Japan
4. Aichi University of Education, Japan
5. Niigata Rehabilitation Hospital, Japan

Citation:

Tamaki H, Yotani K, Ogita F, Hayao K, Kirimto H, Onishi H, Kasuga N, Yamamoto N. Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats. Int J Med Sci 2019; 16(6):822-830. doi:10.7150/ijms.32590. https://www.medsci.org/v16p0822.htm

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Abstract

Electrical stimulation (ES)-induced muscle contraction has multiple effects; however, mechano-responsiveness of bone tissue declines with age. Here, we investigated whether daily low-frequency ES-induced muscle contraction treatment reduces muscle and bone loss and ameliorates bone fragility in early-stage disuse musculoskeletal atrophy in aged rats. Twenty-seven-month-old male rats were assigned to age-matched groups comprising the control (CON), sciatic nerve denervation (DN), or DN with direct low-frequency ES (DN+ES) groups. The structural and mechanical properties of the trabecular and cortical bone of the tibiae, and the morphological and functional properties of the tibialis anterior (TA) muscles were assessed one week after DN. ES-induced muscle contraction force mitigated denervation-induced muscle and trabecular bone loss and deterioration of the mechanical properties of the tibia mid-diaphysis, such as the stiffness, but not the maximal load, in aged rats. The TA muscle in the DN+ES group showed significant improvement in the myofiber cross-sectional area and muscle force relative to the DN group. These results suggest that low-frequency ES-induced muscle contraction treatment retards trabecular bone and muscle loss in aged rats in early-stage disuse musculoskeletal atrophy, and has beneficial effects on the functional properties of denervated skeletal muscle.

Keywords: disuse, osteopenia, muscle force, micro computed tomography

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APA

Tamaki, H., Yotani, K., Ogita, F., Hayao, K., Kirimto, H., Onishi, H., Kasuga, N., Yamamoto, N. (2019). Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats. International Journal of Medical Sciences, 16(6), 822-830. https://doi.org/10.7150/ijms.32590.

ACS

Tamaki, H.; Yotani, K.; Ogita, F.; Hayao, K.; Kirimto, H.; Onishi, H.; Kasuga, N.; Yamamoto, N. Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats. Int. J. Med. Sci. 2019, 16 (6), 822-830. DOI: 10.7150/ijms.32590.

NLM

CSE

Tamaki H, Yotani K, Ogita F, Hayao K, Kirimto H, Onishi H, Kasuga N, Yamamoto N. 2019. Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats. Int J Med Sci. 16(6):822-830.

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