Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Middle Yanchang Road, Jingan District, Shanghai, People's Republic of China
✉ Corresponding author: Yidong Wei MD, Ph.D. Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine. 301 Middle Yanchang Road, Jingan District, Shanghai, People's Republic of China. Telephone number: +86-18917683409 Fax number: 021-66301771 E-mail address: yweiedu.cn. (Yidong Wei).More
Citation:
Li H, Zhao J, Liu B, Luo J, Li Z, Qin X, Wei Y. MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation. Int J Med Sci 2019; 16(1):106-114. doi:10.7150/ijms.28093. https://www.medsci.org/v16p0106.htm
This study shows that microRNA-320 (miR-320) is associated with many important cell functions, including cell differentiation, proliferation, migration, and apoptosis. However, the role of miR-320 in vascular smooth muscle cells (VSMCs) and proliferative vascular diseases is still completely unclear. In our study, we found that the expression of miR-320 in human VSMCs after PDGF stimulation was significantly down-regulated in time- and dose-dependent manner. Function analyses identified that miR-320 could inhibit the proliferation and migration of VSMCs in both basal and PDGF-stimulated conditions. Furthermore, Neuropilin 1 (NRP1) was demonstrated as a direct target of miR-320 in Luciferase reporter assays and miR-320 overexpression inhibited the expression of NRP1 with or without PDGF treatment. Finally, miR-320 was markedly decreased in mice carotid arteries after ligated injury, while the restoration of miR-320 via Ad-miR-320 attenuated neointimal hyperplasia by declining the NRP1 expression. The results confirmed that miR-320 regulated proliferation and migration of VSMCs and neointimal formation by targeting NRP1. These novel findings implied that the regulation of NRP1 expression by miR-320 has important significance in the early diagnosis and treatment of proliferation vascular diseases.
Li, H., Zhao, J., Liu, B., Luo, J., Li, Z., Qin, X., Wei, Y. (2019). MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation. International Journal of Medical Sciences, 16(1), 106-114. https://doi.org/10.7150/ijms.28093.
ACS
Li, H.; Zhao, J.; Liu, B.; Luo, J.; Li, Z.; Qin, X.; Wei, Y. MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation. Int. J. Med. Sci. 2019, 16 (1), 106-114. DOI: 10.7150/ijms.28093.
NLM
Li H, Zhao J, Liu B, Luo J, Li Z, Qin X, Wei Y. MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation. Int J Med Sci 2019; 16(1):106-114. doi:10.7150/ijms.28093. https://www.medsci.org/v16p0106.htm
CSE
Li H, Zhao J, Liu B, Luo J, Li Z, Qin X, Wei Y. 2019. MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation. Int J Med Sci. 16(1):106-114.
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