Int J Med Sci 2018; 15(13):1547-1554. doi:10.7150/ijms.26329
Microarray Analysis of Differentially-expressed MicroRNAs in Acquired Middle Ear Cholesteatoma
1. Department of Otolaryngology-Head and Neck Surgery, The Xiangya Hospital, Central South University, Hunan provincial key lab, Otolaryngology institute of major diseases, Changsha, Hunan 410008, China.
2. Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Xie S, Liu X, Pan Z, Chen X, Peng A, Yin T, Ren J, Liu W. Microarray Analysis of Differentially-expressed MicroRNAs in Acquired Middle Ear Cholesteatoma. Int J Med Sci 2018; 15(13):1547-1554. doi:10.7150/ijms.26329. Available from http://www.medsci.org/v15p1547.htm
Objectives: To analyze the miRNAs expression profiling between acquired middle ear cholesteatoma and normal skin, and to identify several novel miRNAs which may be involved in the etiopathogenesis of middle ear cholesteatoma.
Methods: MiRNA microarray technology was adopted to analyze the miRNA expression profiling between acquired middle ear cholesteatoma and normal skin. qRT-PCR was used to validate selected differentially expressed miRNAs.
Results: The miRNA microarray technology showed totally 44 upregulated (miRNA-21-3p, miRNA-584-5p, miRNA-16-1-3p, etc) and 175 downregulated (miRNA-10a-5p, miRNA-152-5p, miRNA-203b-5p, etc) miRNAs in cholesteatoma tissues with 2-fold change compared with normal skin. The qRT-PCR validation was in accordance with the microarray results partly: miRNA-21-3p and miRNA-16-1-3p expressed significantly higher while miRNA-10a-5p exhibited an obviously decreased expression in middle ear cholesteatoma tissues when compared with normal skin. The GO and KEGG pathway analyses provided clues that these differentially expressed miRNAs might play essential roles in the etiopathogenesis of middle ear cholesteatoma, including cell proliferation, apoptosis, cell cycle, differentiation, bone resorption and remodeling process.
Conclusions: Our study suggests possible roles of differentially expressed miRNAs in the pathogenesis of middle ear cholesteatoma. Targeting on these miRNAs may provide a new strategy for cholesteatoma therapy in the future.
Keywords: miRNA, middle ear, cholesteatoma, etiopathogenesis